条斑紫菜离体组织再生苗的研究  

GENERATION OF Porphyra yezoensis FROM EXCISED FRAGMENT

作　　者：梅俊学[1] 费修绠[1] 

机构地区：[1]中国科学院海洋研究所,青岛264071

出　　处：《海洋科学》2000年第6期39-42,共4页Marine Sciences

基　　金：国家重点科技项目攻关计划资助项目!96 C01 05号

摘　　要：条斑紫菜叶状体离体组织 (切块 )在一定培养条件下 ,其营养细胞经过一系列的变化可产生出幼苗。本研究发现切块越小出苗越早、单位面积离体组织出苗量越多。成熟叶状体上靠近雌雄性细胞的区域与其他部位的营养细胞相比形成幼苗较早 ,离这一区域越远的细胞成苗越晚。离体组织细胞成苗的适宜水温为15～17℃ ,在20～25℃下部分细胞进行不规则分裂形成深紫红色的细胞团 ,当降温至17℃时 ,它们仍可转化为幼苗。Seedlings regenerated from excised tissue of Porphyra yezoensis is a potential resources for the seaweed production. Several factors affecting the seedlings production were studied, that is, temperature, position of the fragments in the thalli, and size of the fragments. The result showed that the fragments regenerated seedlings at 15 22 ℃. At 25 ℃ the tissue cells divided irregularly and produced red cell clumps which did not regenerate seedlings until they were cultured at lower temperature (17 ℃) for 2 weeks. The time needed for seedlings production varied depending on the size and original position of the fragments in the blade. Small fragments (1 mm×1 mm,2 mm×2 mm and 3 mm×3 mm)regenerated sooner than big ones (5 mm×5 mm and 10 mm×10 mm).The tissue from near the reproductive area needed less time for seedlings regenerating than that from just above the holdfast.

关 键 词：条斑紫菜 离体组织 再生苗 紫菜 

分 类 号：Q949.292.1[生物学—植物学] S968.431[农业科学—水产养殖]