桂蚕沙多糖组分的制备与GC-MS分析  

Production of Polysaccharide Components from Faeces Bombycis Gui and Analysis by GC-MS

作　　者：李奇豫[1] 张贵君[1] 李素丽[2] 徐蓓蕾[1] 张雅楠[1] 张智圆[1] 董文茜[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]广西大学农学院,南宁530004

出　　处：《中国实验方剂学杂志》2012年第8期113-115,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科技攻关与新产品试制项目(桂科攻10124008-14)

摘　　要：目的:制备桂蚕沙的多糖并分析其组分,为专属性鉴定特征的建立奠定基础。方法:桂蚕沙多糖制备流程脱脂后水提醇沉→三氯乙酸除蛋白→大孔吸附树脂纯化→活性炭脱色。桂蚕沙多糖组分的确定采用衍生化后GC-MS分析。结果:桂蚕沙多糖提取率为23.5%。桂蚕沙多糖组分:核糖-木糖-鼠李糖-半乳糖-吡喃葡萄糖-阿拉伯糖(1.97∶3.14∶0.58∶0.92∶5.62)。结论:气相色谱-质谱联用技术能够有效的分析桂蚕沙中的多糖组分。Objective：To produce the polysaccharide components from Faeces Bombycis Gui and analyze its composition.Method： Polysaccharide component of Faeces Bombycis Gui was extracted by the methods of water extracting-alcohol precipitating after degreasing,and then removed protein with 10% content of trichloroa cetic acid（TCA）,purified with AB-8 macroreticular resin and decolored by active carbon.Finally,the polysaccharide component after derivatization was analyzed by gas chromatography-mass.Result： The yield of crude polysaccharide could attain 23.5%.The polysaccharide of Faeces Bombycis Gui was composed of ribose,xylose,rhamnose,galactose,glucopyranose and arabinose.The proportion of it was 1.97∶3.14∶0.58∶0.92∶5.62.Conclusion： The GC-MS method could analyze the polysaccharide components of Faeces Bombycis Gui effectively.

关 键 词：桂蚕沙 多糖组分 气相色谱-质谱 专属性鉴定特征 

分 类 号：R284.1[医药卫生—中药学]