茶多酚诱导人胆囊癌细胞GBC-SD凋亡过程中细胞内游离钙离子浓度及线粒体膜电位的变化  

Changes of free calcium ion concentration and mitochondrial membrane potential during apo-ptosis of human gallbladder carcinoma GBC-SD cells induced by tea polyphenols

作　　者：连超群[1] 张静[2] 陈振东[3] 夏俊[1] 

机构地区：[1]蚌埠医学院检验系,安徽蚌埠233030 [2]蚌埠医学院生物科学系,安徽蚌埠233030 [3]蚌埠医学院,2010级研究生安徽蚌埠233030

出　　处：《中国生物制品学杂志》2012年第4期449-452,457,共5页Chinese Journal of Biologicals

基　　金：安徽省教育厅自然科学研究项目(KJ2012B108;KJ2011Z255)

摘　　要：目的探讨茶多酚(Tea polyphenols,TP)诱导人胆囊癌细胞GBC-SD凋亡过程中细胞内游离钙离子浓度[Ca2+]i及线粒体膜电位(△Ψm)的变化。方法采用MTT法检测TP对GBC-SD细胞增殖的影响;Annexin V-FITC/PI双染色,激光扫描共聚焦显微镜(laser scanning cofocal microscopy,LSCM)观察TP对GBC-SD细胞凋亡的影响;Fluo-3 AM和Rhodamine123染色标记,LSCM观察TP对GBC-SD细胞内[Ca2+]i及△Ψm的影响。结果 TP可明显抑制GBC-SD细胞增殖,并诱导细胞凋亡,且呈时间和剂量依赖性;TP能使GBC-SD细胞内[Ca2+]i增加,△Ψm降低,且呈剂量和时间效应关系。结论 TP对GBC-SD细胞具有明显的抑制增殖和诱导凋亡作用,其机制可能与其上调细胞内[Ca2+]i、降低△Ψm有关。[ Abstract] Objective To investigate the changes of free calcium ion concentration （[ Ca2＋]） and mitochondrial membrane potential （△ψm） during apoptosis of human gallbladder carcinoma GBC-SD cells induced by tea polyphenols （TP）. Methods The effect of TP on proliferation of GBC-SD cells was determined by MTr method, while that on apoptosis of GBC-SD cells by laser scanning eofocal microscopy （LSCM） after Annexin V-FITC/PI staining, and that on [Ca2＋]i and △ψm by LSCM after Fluo-3 AM and Rhodamine123 staining. Results TP inhibited the proliferation and induced the apoptosis of GBC-SD cells significantly, both in time- and dose-dependent patterns. Meanwhile, TP increased the [Ca2＋]i and decreased △ψm, both in time- and dose-dependent patterns. Conclusion TP inhibited the proliferation and induced the apoptosis of GBC-SD cells by a possible mechanism of up- regulating the intracellular [ Ca2＋]i and decreasing the △ψm.

关 键 词：茶多酚 胆囊癌 GBC-SD细胞 钙离子浓度 线粒体膜电位 

分 类 号：Q257[生物学—细胞生物学]