海参岩藻聚糖硫酸酯抗肿瘤转移作用研究  

Inhibitory effects of fucoidan,a sulfated polysaccharides from Cucumaria frondosa on experimental metastasis in mice

作　　者：王静凤[1] 张珣[1] 李辉[1] 杨玉红[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国海洋药物》2012年第2期14-18,共5页Chinese Journal of Marine Drugs

基　　金：海洋公益性行业科研专项(No.201105029);国际科技合作项目(No.2010DFA31330);国家自然科学基金(No.30972284);"泰山学者"建设工程专项经费;教育部博士点基金(200804230008)

摘　　要：目的建立小鼠黑色素瘤B16细胞实验性肺转移模型,探讨海参岩藻聚糖硫酸酯(sea cucumber fu-coidan,SC-FUC)的体内抑制肿瘤肺转移作用。方法连续腹腔注射SC-FUC 26d后,检测小鼠肺转移灶数量、血清中唾液酸的含量、γ-谷氨酰转肽酶活力和肺组织中羟脯氨酸、氨基己糖、糖醛酸的含量。结果SC-FUC剂量组小鼠的肺转移灶数量显著减少(P<0.01),平均转移抑制率为65.25%,血清唾液酸含量和γ-谷氨酰转肽酶活力显著降低(P<0.01),肺组织中羟脯氨酸、氨基己糖、糖醛酸的含量显著下降(P<0.01)。结论SC-FUC能显著抑制肿瘤细胞在小鼠体内的转移和生长。Objective In the present study, the experimental metastatic mouse model was established by intravenous injection of B16-F10 melanoma cells and the anti-metastatic effect of SC-FUC was investigated. Methods Forty mice were randomly selected and assigned into four groups （ten animals per group） the normal control group, the model control group, low and high dosage groups （50 and 100 mg kg-1 bw）. Each mouse was treated daily by intraperitoneally injection of SC-FUC or normal saline before tumor inoculation for 5 days. On the 6th day, B16-F10 melanoma cells were transferred intravenously into the tail vein of each mouse except for the normal control group. The mice were sacrificed after 20 days of tumor induction, blood was collected for separating the serum and the lungs were dissected out to detect metastatic colonies. Levels of serum sialic acids, y-glutamyl transpeptidase and hydroxyproline, hexosamines, uronic acid in the lung tissues were measured respectively. Results SC-FUC inhibited the metastases formation by about 65.25% as compared to model control animals. SC-FUC significantly reduced the concentrations of serum sialie acids content and y-GT energy （P〈0.01）, which reflected occurrence, development, metastasis and curative effect of the lung cancer to certain extent. Biochemical parameters such as hydroxyproline, hexosamines and uronic acid levels were also reduced clearly （P%0.01） in the SC-FUC-treated animals. Conclusion SC-FUC can inhibit the experimental metastasis of B16-F10 melanoma cells in C57BL/6 mice.

关 键 词：海参 多糖 抗肿瘤 Γ-谷氨酰转肽酶 羟脯氨酸 氨基己糖 

分 类 号：R931.7[医药卫生—生药学] R965[医药卫生—药学]