纤维素降解细菌筛选及降解特性分析  

Study on the screening of cellulose-degrading bacteria and the degradation characteristics

作　　者：刘乐[1] 鞠美庭[1] 李维尊[1] 江洋[1] 

机构地区：[1]南开大学环境科学与工程学院,天津300071

出　　处：《环境污染与防治》2012年第4期40-43,48,共5页Environmental Pollution & Control

摘　　要：基于获得高效纤维素降解细菌的目的,通过LB培养基的培养以及刚果红培养基的筛选,从牛粪堆肥中筛选获得2株高效纤维素降解细菌。经鉴定,分别为枯草芽胞杆菌(Bacillus subtilis)和地衣芽胞杆菌(Bacillus licheniformis)。所筛选得到的菌种具有很高的滤纸降解能力,可在6d内使滤纸剧烈崩溃,振摇成均匀糊状;其中,地衣芽胞杆菌的羧甲基纤维素钠酶活峰值在发酵第4天达到峰值(237U/g)。In order to obtain high efficient cellulose degrading bacteria,LB medium and Congo red agar medium were used to culture and screening strains that isolated from cow dung compost.Results showed that the two obtained strains were identified as Bacillus subtilis and Bacillus licheniformis.In the filter paper decomposition experiment,the filter paper could be shaken into paste in 6 days,which indicated that the two strains had great capacity of filter paper decomposition.The sodium carboxy methyl cellulose enzyme activity of Bacillus licheniformis reached the peak value（237 U/g） at the fourth day of fermentation.

关 键 词：分离筛选 纤维素降解 酶活 

分 类 号：X172[环境科学与工程—环境科学]