近红外光谱法同时测定通天口服液中天麻素和芍药苷的含量  

Simultaneous Determination of Gastrodin and Paeoniflorin in Tongtian Oral Liquid by Near-infrared Spectroscopy

作　　者：蒙杰丹[1] 刘冰[1] 杨琼[2] 周尚[2] 杨季冬[1,2] 

机构地区：[1]西南大学化学化工学院,重庆北碚400715 [2]长江师范学院化学化工学院,重庆涪陵408100

出　　处：《分析科学学报》2012年第2期197-201,共5页Journal of Analytical Science

基　　金：重庆市科委科技攻关项目(No.2010AC5170);涪陵区科委攻关项目(No.2011ABA2028)

摘　　要：本文应用近红外光谱结合偏最小二乘法建立了同时测定通天口服液中天麻素与芍药苷含量的方法。以高效液相色谱(HPLC)法测定通天口服液样品中天麻素和芍药苷的化学参考值,随机抽取60个样本作校正集,20个样本作预测集。用偏最小二乘法(PLS)将校正集样本的近红外光谱与相应样本的天麻素和芍药苷含量分别相关联建立模型。结果表明,天麻素和芍药苷校正模型的决定系数分别为96.28%、94.55%,模型的交叉验证均方差分别为0.0336、0.00908,预测集的决定系数分别为94.23%、92.86%,预测集均方差分别为0.0453、0.00839。同时还做了模型的精密度实验,该方法能用于大批量样品的快速分析。A new,rapid analytical method for the analysis of gastrodin and paeoniflorin in Tongtian oral liquid by near infrared spectroscopy combined the partial least square method was developed.The content of gastrodin and paeoniflorin in the Tongtian oral liquid samples was determined by high performance liquid chromatography（HPLC）,and 60 samples were randamly chosen for calibration set,while other 20 samples were used for prediction set.The associated models of the near infrared spectroscopy in calibration set and the corresponding content of gastrodin and paeoniflorin were established by the partial least square method,respectively.The results showed that the coefficients of determination of calibration models were 96.28% and 94.55%,and the root mean squared errors of corss-validation were 0.0336 and 0.00908,respectively.The coefficients of determination of the prediction models were 94.23% and 92.86%;and the root mean squared errors of prediction were 0.0453 and 0.00839,respectively.Some precisiom expeiments were also performed with satisfying results.The method was accurate,rapid and simple.It can be applied for the rapid determination of large numbers of samples.

关 键 词：近红外光谱 通天口服液 天麻素 芍药苷 

分 类 号：O657.33[理学—分析化学]