高脂诱导胰岛素抵抗apoE^(-/-)小鼠HMGCR、SREBP-2和LDLr基因表达的变化  

Changes of HMGCR,SREBP-2 and LDLr mRNA expression in high-fat induced insulin resistance apoE^(-/-)mice

作　　者：汤世国[1] 李伶[2] 杨刚毅[1] 孙滨[2] 李钶[1] 晏丕军[1] 李龙辉[1] 董靖[1] 吴丹冬[1] 

机构地区：[1]重庆医科大学附属第二医院内分泌科,400010 [2]重庆医科大学检验系临床生化教研室和教育部实验诊断重点实验室

出　　处：《中国糖尿病杂志》2012年第4期292-294,共3页Chinese Journal of Diabetes

基　　金：国家自然科学基金项目(30771037;30871199;30971388)

摘　　要：目的探讨高脂诱导IR apoE-/-小鼠胆固醇代谢基因的变化。方法健康雄性apoE-/-小鼠,随机分为普食组(NF组)和高脂喂养组(HF组),共喂养16周。采用实时荧光定量PCR测定肝组织羟甲基戊二酸单酰辅酶A还原酶(HMGCR)、固醇调节元件结合蛋白-2(SREBP-2)、低密度脂蛋白受体(LDLr)基因mRNA表达。结果 HF组小鼠FBG、FIns、TC、TG、HDL-C、LDL-C和FFA水平均明显升高(P均<0.01)。HF组小鼠肝脏HMGCR、SREBP-2和LDLr mRNA表达水平均明显降低(P<0.01或P<0.05)。结论在高脂喂养apoE-/-小鼠,HMGCR、SREBP-2和LDLr的变化在其胆固醇代谢紊乱中可能具有重要作用。Objective To investigate the effects of high-fat induced insulin resistance （IR） on gene- related cholesterol metabolism in apoE-/- mice. Methods Healthy male apoE/- mice were randomized into normal diet group （NF group, n= 10） and high-fat group （HF group, n=10） and were fed for 16 weeks. The mRNA expressions of 3-hydroxy-3-methylglutaryl eoenzyrae A reductase （HMGCR）, sterol regulatory element binding protein-2 （SREBP-2）, and low density lipoprotein receptor （LDLr） of the hepatic tissues were measured by quantitative real-time PCR. Results Compared with the NF group, the fasting blood glucose （FBG）, fasting plasma insulin （Fins）, total cholesterol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, and free fatty acid （FFA） were significantly increased （all P^0. 01）, while the mRNA expression of HMGCR, SREBP- 2, and LDLr were significantly decreased in the hepatic tissues of apoE-/- mice （P〈0. 01 and P〈0. 05） in the HF group. Conclusion The down-regulation of HMGCR, SREBP-2 and LDLr might contribute to the disorder of cholesterol metabolism in high-fat fed apoE/- mice.

关 键 词：APOE-/-小鼠 胰岛素抵抗 胆固醇 基因 

分 类 号：R363[医药卫生—病理学]