槲皮素增强乳腺癌细胞化疗敏感度的研究  

Quercetin Sensitizes Breast Cancer Cells Chemotherapy in Vitro

作　　者：李世正[1] 李昆[2] 张俊华[1] 董哲[1] 

机构地区：[1]辽宁医学院附属第一医院普外科,辽宁锦州121001 [2]辽宁医学院附属第一医院检验科,辽宁锦州121001

出　　处：《肿瘤防治研究》2012年第4期381-388,共8页Cancer Research on Prevention and Treatment

摘　　要：目的探讨槲皮素(quercetin,Que)对乳腺癌细胞化疗药物敏感度的影响及其可能的机制。方法 MTT法检测不同浓度Que对人乳腺癌MCF-7细胞生长的影响,单用阿霉素(adriamycin,ADR)或ADR联合无毒剂量Que作用于人乳腺癌MCF-7细胞,MTT法检测药物对细胞的增殖抑制作用,流式细胞法检测细胞凋亡率;Transwell小室法检测细胞侵袭能力的变化;RT-PCR法及Western blot法分别检测缺氧诱导因子-1α(HIF-1α)mRNA水平和蛋白水平的表达变化。结果不高于0.7μM Que对细胞生长无影响。与单用ADR组相比较,0.7μM Que与ADR联用可提高ADR对人乳腺癌MCF-7细胞的增殖抑制率、诱导其细胞凋亡、降低其体外侵袭和转移能力、下调HIF-1αmRNA和蛋白的表达。结论 Que能够增强人乳腺癌MCF-7细胞化疗敏感度,其机制可能与下调该细胞HIF-1α的mRNA和蛋白表达有关。Objective To study the effects and its mechanism of Que on the chemotherapeu-tics sensitivity of human breast cancer cells. Methods The effect of Que on the growth of human breast cancer MCF 7 cells was examined by MTT assay. MCF 7 cells were treated by ADR with or without non-cytotoXic dose of Que. Then, the proliferative capacity of the cells was measured using MTT assay, the cell cycle was an alysed using flow cytometry, the invasive capacity was measured by transwell chamber invasive model, HIF la mRNA level was determined by RT PCR,and HIF la protein expression was detected by West- ern blot assay. Results Less than 0. 7 μM Que had non-cytotoxic effect on the growth MCF-7 cells. Compared with ADR alone action on MCF-7 cells, 0. 7 μM Que could enhance inhibitory rate of cell growth,more apoptotie cells,inhibitory capability,and expression of HIF-lα mRNA and protein with the same dose of ADR. Conclusion Que has potential application in the treatment of chemotherapy-resistant- breast cancer.

关 键 词：槲皮素 乳腺癌 化疗增敏 

分 类 号：R737.9[医药卫生—肿瘤]