黄芪、水蛭、大黄及其复方含药血清影响大鼠MC产生FN及Col-Ⅳ的研究  

Research on Huangqi Shuizih and Dahuang and Their Compounds on Rats MC Secreteing FN and Col-Ⅳ

作　　者：翟文生[1] 白洁[1] 王文英[1] 郭会敏[1] 刘华[1] 朱珊 高旭光[1] 赵小静[1] 平东沛[1] 

机构地区：[1]河南中医学院第一附属医院,河南郑州450000 [2]河南中医学院第二附属医院,河南郑州450011

出　　处：《辽宁中医杂志》2012年第4期739-741,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：河南省杰出青年计划(084100510018)

摘　　要：目的:研究黄芪、水蛭、大黄及其复方抑制大鼠系膜细胞(mesangial cells,MC)产生纤维连接蛋白(fibronec-tin,FN)及Ⅳ型胶原(typeⅣcollagen,Col-Ⅳ)的强弱。方法:在大鼠MC体外培养的基础上,将脂多苷糖(lipopolysac-charide,LPS)刺激的MC增生模型分为9组:控制组、模型组、对照组、黄芪组、水蛭组、大黄组、雷公藤多苷组、复方组、复方+雷公藤多苷组。采用ELISA法检测黄芪、水蛭、大黄及其复方等含药血清对MC表达FN及Col-Ⅳ的影响。结果:各含药血清组均能明显降低经LPS刺激后的大鼠MC产生FN及Col-Ⅳ的量,以复方+雷公藤组作用最佳,水蛭组、大黄组抑制作用较强,优于黄芪组。结论:证实了化瘀、泄浊中药水蛭、大黄在抑制MC产生FN及Col-Ⅳ方面优于益气类中药黄芪,复方+雷公藤组作用效果最佳。Objective：To research the effect of Huangqi, Shuizhi, Dahuang and their compounds inhibiting rat mesangium cells to produce fibronectin and type IV collagen. Methods ： On the basis of rat mcsangial cells culture in vitro, the rat mesangial cells hyperplasia model was induced by LPS into nine groups ： control group, model group, matched group, Huangqi group, Shuizhi group, Dahuang group,tripterygium wilfordii group, compounds group, Compound ＋ tripterygium wilfordii group. ELISA method was used to test expressions of FN and Col - IV by MC, which were influenced by Huangqi, Shuizhi, Dahuang and their compounds. Results ： Each serum containing medicine group significantly lowered producations of FN and Col - IV by rat MC which were stimulated by LPS. Shuizhi group＇s inhibition is better than Huangqi group＇s. Conclusion：It is confirmed Shuizhi and Dahuang were better than Huangqi in inhibiting rat mesangium cells to produce fibronectin and type IV collagen, and the effect of com- pound ＋ tripterygium wilfordii group was the best.

关 键 词：系膜增生性肾小球肾炎 系膜细胞 含药血清 FN Col-Ⅳ 

分 类 号：R-322[医药卫生]