ZnPc和HPD介导的PDT对肺癌细胞杀伤效果的对比研究  

The Study of Comparing the Efficiency of ZnPC-PDT and HPD-PDT in Killing LLC Lung Cancer

作　　者：李维娜[1] 杨继庆[1] 刘渊声[1] 文峻[1] 屈学民[1] 龙开平[1] 

机构地区：[1]第四军医大学,陕西西安710032

出　　处：《中国医学物理学杂志》2012年第2期3289-3292,3304,共5页Chinese Journal of Medical Physics

基　　金：国家自然科学基金资助项目(No.10804129)

摘　　要：目的:对比不同光敏剂浓度、不同光照时间和不同激光功率密度下,ZnPc和HPD对肺癌细胞的杀伤效果,探索了ZnPc-PDT的最佳工作参数。方法:分别选用ZnPc和传统光敏剂HPD为研究对象,使其作用于Lewis肺癌细胞,通过分光光度计测量其吸收峰值,选取合适的激发光源;通过倒置显微镜和MTT法的检测,对比了不同光敏剂浓度、不同光照时间及不同激光功率密度下ZnPC-PDT和HPD-PDT对LLC肺癌细胞的杀伤能力。结果:(1)ZnPc的吸收峰的波峰较HPD相对单一,且波长较长;(2)ZnPc和HPD对肺癌细胞的杀伤作用均随光敏剂浓度、光照时间、光源功率密度的增强而增强,但增大到一定值后会趋于饱和;(3)相同条件下,ZnPc的杀伤效果均强于HPD,且具有显著性差异。结论:肺癌治疗方面ZnPc-PDT是一种较HPD-PDT更为高效的治疗手段。Objective： To compare the efficiency of two photosensitizers ZnPC and HPD in killing LLC lung cancer under different concentration, exposure time and power density. Explore the best parameters of ZnPc-PDT for lung cancer. Methods： This artcle selects the second generation photosensitizer ZnPc and traditional photosensitizer HPD as the research objects, applied them to the LLC lung cancer cells, uses ultraviolet spectrophotometer to measure the absorption peak values and selectes proper excitation light resources; uses inverted microscope and MTT to observe and compare the killing effects of ZnPC-PDT and HPD-PDT on LLC lung cancer cells under different photosensitizers＇ concentration, exposure time, and different laser power density. Results： （1） Comparing to HPD, ZnPc has a singular absorption wave peak with longer wavelength. （2）In both cases, the effects of killing lung cancer increases with the increasing ofphotosensitizers＇ concentration, exposure time, and different light source power density, while the effects would be saturated after reaches certain value. （3）At the same conditions, ZnPc is more effective in killing lung cancer than HPD with distinctive difference. Conclusions： ZnPc-PDT is a more effective treatment in curing lung cancer than HPD-PDT

关 键 词：光动力学疗法 光敏剂 肺癌 

分 类 号：R774[医药卫生—眼科]