极低频率电磁场对肝癌细胞SODD和Survivin基因表达的影响  

Effects of ELF on gene expression of SODD and Survivin in hepatoma cell lines

作　　者：张琰[1] 刘雅恬[1] 赵炎斌[1] 寇莹莹[2] 刘宇飞[1] 吴剑秋[1] 

机构地区：[1]江苏省肿瘤医院内科,江苏南京210009 [2]中国药科大学基础部,江苏南京210009

出　　处：《中国肿瘤外科杂志》2012年第2期101-104,共4页Chinese Journal of Surgical Oncology

摘　　要：目的研究极低频率电磁场(extremely low frequency electromagnetic field,ELF)对肝癌细胞SODD和Survivin基因表达的影响,探索ELF在治疗肝癌中的作用。方法通过RT-PCR和流式细胞仪检测经ELF处理过的肝癌细胞系BEL-7402与正常肝细胞系L-02中SODD和Survivin基因表达的情况,分析ELF对肝癌细胞SODD和Survivin基因表达的影响。结果经ELF处理后,BEL-7402细胞中SODD和Survivin基因表达明显下降,而对L-02细胞没有影响。结论 ELF能够促进抑制凋亡基因SODD、Survivin在肝癌细胞中的表达下调,提示ELF在促进肝癌细胞凋亡方面具有重要作用,为ELF在临床治疗肝癌提供了线索。Objective To examine the effects of ELF on gene expression of SODD and Survivin in hepatoma cell lines,which may indicate the role of ELF in the treatment of liver cancer.Methods The hepatoma cell lines BEL-7402 and L-02 liver cells were treated by ELF and gene expression of SODD and Survivin were analyzed by RT-PCR and FCM,in which L-02 liver cells was served as control.Results The results of RT-PCR showed that the expressions of SODD and Survivin went down in hepatoma cell lines and didn＇t change in L-02 liver cells after the treatment of ELF.Conclusions ELF has effects on the down-regulwtion of gene expression of SODD and Survivin in the hepatoma cell lines,indicating that ELF may facilitate apoptosis in the hepatoma cell lines.Therefore,ELF may play a role in the treatment of liver cancer in further.

关 键 词：肝细胞癌 SODD SURVIVIN 基因表达 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]