BATF2对Wnt信号通路影响的研究  

The Investigation of Influence on Wnt Signaling Pathway by BATF2

作　　者：郑水娣[1] 吴帅[2] 桂镜华[1] 贾晓渊[1] 章康健[2] 

机构地区：[1]浙江理工大学生命科学学院新元医学与生物技术研究所,杭州310018 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《中国细胞生物学学报》2012年第4期343-348,共6页Chinese Journal of Cell Biology

基　　金：浙江理工大学研究生创新基金(No.YCX-S11021);浙江理工大学科研启动基金(No.1016834-Y;No.1016845-Y);浙江省自然科学基金(No.Y2090935)资助项目~~

摘　　要：Wnt信号通路在各种生物体内高度进化保守,与癌症的发生发展密切相关。BATF2是一个新近发现的基因,研究表明其具有抑癌基因的作用。目前,BATF2与Wnt信号通路的关系尚不清楚,该文用荧光素酶报告基因检测、Real-time PCR和Western blot发现BATF2能影响Wnt信号通路。过表达BATF2可明显下调TCF4/β-catenin的转录活性和Wnt信号通路下游基因的表达,可以下调细胞核中的β-catenin。推测BATF2可能通过下调细胞核中的β-catenin来实现对Wnt信号通路的下调。上述结果为抑制Wnt信号通路用于肿瘤治疗提供了一定的依据。Abstract Wnt signal pathway is highly conserved in different species throughout evolution, which plays a crucial role in tumor genesis and progression. BATF2 is a novel gene, which has shown the antitumor activity. But the relationship between Wnt pathway and BATF2 has not been clearly investigated. In this study, we revealed that BATF2 could influence the Wnt signaling pathway by luciferase report assay, Real-time PCR and Western blot. The overexpression of BATF2 could significantly reduce the TCF4/β-catenin transcriptional activity and the Wnt target genes expression, and downregulate the β-catenin in the nuclear. We hypothesized that the BATF2 reduced the Wnt signaling pathway through downregulating β-catenin in nuclear. The results provided an antitumor therapy through inhibit Wnt signaling pathway.

关 键 词：BATF2 WNT信号通路 TCF4/β-catenin转录活性 Β-CATENIN 

分 类 号：R730.5[医药卫生—肿瘤]