胆汁酸对胃癌细胞株MKN-28生长的影响  

Effect of Bile Acids on the Growth of Gastric Cancer Cell Line MKN-28

作　　者：王学伟[1] 曹勤[1] 

机构地区：[1]上海中医药大学附属普陀医院消化内科,200062

出　　处：《胃肠病学》2012年第3期176-178,共3页Chinese Journal of Gastroenterology

基　　金：上海中医药大学附属普陀医院科研项目(No.20075030A)资助

摘　　要：胆汁反流是常见现象，其对胃上皮细胞生长的影响尚不明确。目的：探讨胆汁酸体外对胃癌细胞株MKN-28生长的影响。方法：采用Mr丌法测定不同浓度牛磺石胆酸钠（TLC）和甘氨鹅脱氧胆酸钠（GCDC）对胃癌细胞株MKN．28生长的影响。结果：第1-6d，60-300μmol／LTLC可明显抑制MKN-28细胞生长，且呈剂量依赖性。300μmol／LGCDC干预24h可显著抑制MKN-28细胞生长。结论：脂溶性胆汁酸TLC和水溶性胆汁酸GCDC体外均可直接抑制胃癌细胞生长。Bile reflux is a common phenomenon and its effect on the growth of gastric epithelium cells is still unknown. Aims： To assess the effect of bile acids on the growth of gastric cancer cell line MKN-28 in vitro. Methods： The growth of gastric cancer cell line MKN-28 under the intervention of various concentrations of taurolithocholate （TLC） and glycochenodeoxyeholate （GCDC） was assessed by MT1 method. Results： From day 1 to day 6, 60-300 Ixmol/L TLC exhibited significant inhibitory effect on the growth of MKN-28 cell in a dose-dependent manner. GCDC 300 Ixmol/L exhibited significant inhibitory effect on the growth of MKN-28 cell after 24 hours of intervention. Conclusions： Either the fat-soluble TLC or the water-soluble GCDC can directly inhibit the growth of gastric cancer cell in vitro.

关 键 词：胆汁酸类和盐类 牛磺石胆酸钠 甘氨鹅脱氧胆酸钠 上皮细胞 生长 

分 类 号：R735.2[医药卫生—肿瘤]