两歧双歧杆菌产生物膜培养基的优化(英文)  

Statistical optimization of medium composition for biofilm formation by Bifidobacterium bifidium

作　　者：张大革[1] 徐文生[1] 张艳艳[1] 黄漫青[1] 王晓东[1] 高秀芝[1] 易欣欣[1] 张磊[1] 艾启俊[1] 

机构地区：[1]农产品有害微生物及农残安全检测与控制北京市重点实验室,北京农学院食品科学与工程学院,北京102206

出　　处：《北京农学院学报》2012年第1期60-64,共5页Journal of Beijing University of Agriculture

基　　金：北京市教委资助项目"双歧杆菌群体感应基因的研究"(KM200910020011);北京市教委重点学科建设资助项目(PXM2009-014207-078172);北京市教委本科生实践教学体系构建项目(PXM2011_014207-000059)

摘　　要：通过数理统计的方法优化双歧杆菌产生物膜培养基。试验首先采用Plackett-Burman试验确定NaCl、MnSO4和半乳糖为影响双歧杆菌生物膜生成的主要因素,再利用正交试验和中心组合试验确定最佳浓度。结果表明:MnSO4、半乳糖和NaCl的最佳浓度分别为0.005g/L,25g/L和0.1mol/L。The medium for biofilm formation by B. bifidium was optimized by statistical methodology for the first time. Two-level factorials of Plackett-Burman design was applied to evaluate the influence of related nutrients on biofilm formation. The sodium chloride, manganese sulfate and galactose were confirmed as critical factors in the medium. The orthogonal design was used to determine the optimal region of these factors for biofilm formation. Then the optimized level of these factors was deter-mined by response surface methodology. The predicted maximal biofilm formation appeared at the region where the concentrations of manganese sulfate, galaetose and sodium chloride were 0. 005 g/L, 25 g/L and 0.1 mol/L, respectively. The correlation between predicted value and measured value of these experiments proved the validity of the response model. The biofilm formation increased 1.5 times in the optimized medium compared to that in the original medium.

关 键 词：双歧杆菌 生物膜 培养基 

分 类 号：TS201[轻工技术与工程—食品科学]