WT-1通过下调c-myb表达抑制U937细胞增殖  

WT1 INHIBITED PROLIFERATION OF HUMAN MONOCYTIC U937 CELLS BY DOWNREGULATING C-MYB EXPRESSINO

作　　者：卢青 刘革修 欧大明 曾高峰 

机构地区：[1]衡阳医学院附属二医院血液内科,421001 [2]衡阳医学院药理教研室 [3]衡阳医学院附属一医院血液科

出　　处：《中国现代医学杂志》2000年第5期8-10,共3页China Journal of Modern Medicine

摘　　要：:研究Wilms瘤基因在白血病发病中的作用机制。方法 :采用反义、Northernblot和流式细胞技术分析了硫代反义WT - 1基因脱氧寡核苷酸和硫代反义c -myb基因对U937细胞增殖和基因表达的影响。结果 :5 μmol/LWT1反义脱氧寡核苷酸处理的U937细胞WT1蛋白表达下降 37% ,c-mybmRNA水平上升不明显 ;10 μmol/L时可抑制其WT1蛋白 87% ,c -mybmRNA水平上升明显 ,同时Myb蛋白水平上升 46 % (P<0 .0 1)。 5 ,10 μmol/Lc -myb基因反义脱氧寡核苷酸可抑制其Myb蛋白 39%、83% (与对照组比较 ,均P <0 .0 1) ,对WT1表达无显著改变。结论 :WT1可以通过下调cObjective: To elucidate a role for the Wilms tumor gene (WT1) in leukemogenesis. Methods: We investigated the effect of phosphorothioate antisense oligodeoxynucleotides (AN-S-ODNs) specific to WT1 gene on the expression of c-myb gene in U937 cells. Northern blot and flow cytometry technologies were used to detect mRNA and protein levels, respectively. Results: 5umol/L AN-S-ODNs for WT1 mRNA significantly inhibited WT1 protein expression in U937 cells at 37% (p<0.01). 10 umol/L AN-S-ODNs inhibited WT1 protein expression in U937 cells at 87% (p<0.01). And increased c-myb expression both at mRNA and protein level (46%, p<0.01), but no effect on WT1 expression in U937. Conclusions: Wilms tumor gene regulates the expression of c-myb gene expression in U937 cells.

关 键 词：U937细胞 Wilms瘤基因 脱氧寡核苷酸 白血病 

分 类 号：R733.7[医药卫生—肿瘤]