登革2型病毒04株5′和3′末端的序列分析  

Sequence analysis of the 5′ and 3′ terminal regions of dengue type 2 virus 04 strain

作　　者：杨敬[1] 杨佩英[1] 秦鄂德[1] 胡志君[1] 于曼[1] 欧武[1] 仝莉莉[1] 赵卫[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病毒室,北京100850

出　　处：《中华实验和临床病毒学杂志》2000年第1期24-26,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　测定登革 2型病毒 0 4株 (D2 0 4)基因组 5′和 3′末端序列。方法　从D2 0 4感染的C6 / 36细胞中提取总RNA ,以该RNA为模板 ,利用RACE法 ,分别扩增D2 0 4株的 5′和 3′末端cDNA片段。将其分别与 pGEM T载体连接得到含有 5′端 5 35bp和 3′端 5 0 3bpcDNA的重组质粒 ,并测定上述cDNA插入片段的序列。将D2 0 4的 5′和 3′端非编码区的核苷酸序列与其它登革 2型毒株进行同源性比较。结果　D2 0 4株与JAM、NGC、S1、16 6 81和PDK 5 3株的同源性分别为98 96 % ,98 96 % ,93 75 % ,98 95 % ,97 92 %和 97 72 % ,97 80 % ,90 6 5 % ,94 2 6 % ,94 2 2 %。结论　D2 0 4株除与S1株的同源性略低外 ,其余株的同源性均在 94%以上。Objective To analize the sequences of 5′ and 3′ terminal region of dengue type 2 virus 04 strain. Methods Total RNA was isolated from dengue type 2 virus 04 strain (D2 04) infected C6/36 cells. With this RNA as templete, the cDNA of both 5′ and 3′ termini of D2 04 were amplified using RACE method respectively. The cDNAs were separately inserted into pGEM T vector and the recombinant plasmids containing the 5′ terminus 535 bp and 3′ terminus 503 bp were obtained. The nucleotide sequence of the inserted cDNA fragment were determined. The nucleotide sequences of the 5′ and 3′ noncoding regions of D2 04 were compared with other Dengue type 2 viruses such as JAM、NGC、S1、16681 and PDK 53. Results The results showed that they shared 98 96%,98 96%,93 75%,98 95%,97 92% and 97 72%,97 80%,90 65%,94 26%,94 22% homology with D2 04 strain respectively. Conclusion Except S1, the homology between D2 04 and other type 2 viruses was higher than 94%.

关 键 词：登革热病毒 基因 序列分析 

分 类 号：R373[医药卫生—病原生物学]