病毒性肝炎肝细胞凋亡及与肝纤维化的关系  

Study on relationship between the apoptosis of hepatocytes and liver fibrosis of chronic viral hepatitis

作　　者：辛绍杰[1] 赵景民[1] 王林杰[1] 王松山[1] 

机构地区：[1]解放军302医院三科,北京100039

出　　处：《中华实验和临床病毒学杂志》2000年第1期31-33,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　研究病毒性肝炎肝细胞凋亡及与肝纤维化的关系。方法　以原位末端标记及免疫组化检测 40例慢性病毒性肝炎 (CH)肝组织细胞凋亡相关线状断裂DNA以及Fas抗原、转化生长因子 β1(TGF β1)、Ⅲ型前胶原肽 (PⅢP)在肝组织中的表达 ;以酶联免疫吸附测定 (ELISA)检测血清可溶性Fas(sFas)及TGF β1。 结果　CH肝细胞DNA损伤与肝组织Fas抗原、TGF β1表达及血清sFas、TGF β1水平明显相关 (r =0 6 2 19,0 5 36 8,0 5 5 6 4,0 5 996。P <0 0 5～ 0 0 1) ;重度CH及肝硬变患者DNA损伤程度明显高于轻度CH患者 ,且与肝组织PⅢP、TGF - β1的表达明显相关。结论　sFas可能也是促进肝细胞凋亡的因素之一。在肝细胞凋亡的同时伴有胶原等细胞外基质合成的增加 ,而TGF β1在细胞凋亡与肝纤维化之间可能起着极为重要的桥梁作用。Objective To study the relationship between apoptosis of hepatocytes and liver fibrosis of chronic viral hepatitis(CH). Methods DNA fragmentation, one of the characteristic features of apoptosis,was detected by in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL) and the expression of Fas, transforming growth factor β1(TGF β1) and Pre collagen Ⅲ peptide(P Ⅲ P)in liver tissues were detected by immunohistochemistry. Soluble Fas (sFas) and TGF β1 in serum were detected by ELISA. Results The damage of DNA in hepatocytes of CH correlated closely with the expression of Fas and TGF β1 in liver tissue and serum( r =0 6129,0 5368,0 5564,0 5996; P <0 05～0 01). In severe type CH and liver cirrhosis, the degree of liver fibrosis and the expression of P Ⅲ P and TGF β1 were higher than that in mild type and moderate type CH. Conclusion The sFas in serum may be one of the factors associated with the apoptosis. The apoptosis of hepatocytes correlated with liver fibrosis of CH and TGF β1 may play an important role between apoptosis and liver fibrosis.

关 键 词：脱噬作用 肝硬化 病毒性肝炎 病理 

分 类 号：R363[医药卫生—病理学]