Identification of naphthalene metabolism by white rot fungus Armillaria sp.F022  

作　　者：Tony Hadibarata Abdull Rahim Mohd Yusoff Azmi Aris Risky Ayu Kristanti 

机构地区：[1]Institute of Environmental and Water Resources Management,Universiti Teknologi Malaysia,81310 Skudai,Johor,Malaysia [2]Department of Chemical and Environmental Engineering.Yamanashi University,4-4-37 Takeda,Kofu,Yamanashi,400-8510 Japan

出　　处：《Journal of Environmental Sciences》2012年第4期728-732,共5页环境科学学报（英文版）

基　　金：supported by Research University Grant of Universiti Teknologi Malaysia(No. Q.J13000.7122.00J31)

摘　　要：Armillaria sp. F022, a white rot fungus isolated from tropical rain forest （Samarinda, Indonesia） was used to biodegrade naphthalene in cultured medium. Transformation of naphthalene by Armillaria sp. F022 which is able to use naphthalene, a two ring-polycyclic aromatic hydrocarbon （PAH） as a source of carbon and energy was investigated. The metabolic pathway was elucidated by identifying metabolites, biotransformation studies and monitoring enzyme activities in cell-free extracts. The identification of metabolites suggests that Armillaria sp. F022 initiates its attack on naphthalene by dioxygenation at its C-1 and C-4 positions to give 1,4-naphthoquinone. The intermediate 2-hydroxybenzaldebyde and salicylic acid, and the characteristic of the meta-cleavage of the resulting diol were identified in the long-term incubation. A part from typical metabolites of naphthalene degradation known from mesophiles, benzoic acid was identified as the next intermediate for the naphthalene pathway of this Armillaria sp. F022. Neither phthalic acid, catechol and cis, cis-muconic acid metabolites were detected in culture extracts. Several enzymes （manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase） produced by ArmiUaria sp. F022 were detected during the incubation.Armillaria sp. F022, a white rot fungus isolated from tropical rain forest （Samarinda, Indonesia） was used to biodegrade naphthalene in cultured medium. Transformation of naphthalene by Armillaria sp. F022 which is able to use naphthalene, a two ring-polycyclic aromatic hydrocarbon （PAH） as a source of carbon and energy was investigated. The metabolic pathway was elucidated by identifying metabolites, biotransformation studies and monitoring enzyme activities in cell-free extracts. The identification of metabolites suggests that Armillaria sp. F022 initiates its attack on naphthalene by dioxygenation at its C-1 and C-4 positions to give 1,4-naphthoquinone. The intermediate 2-hydroxybenzaldebyde and salicylic acid, and the characteristic of the meta-cleavage of the resulting diol were identified in the long-term incubation. A part from typical metabolites of naphthalene degradation known from mesophiles, benzoic acid was identified as the next intermediate for the naphthalene pathway of this Armillaria sp. F022. Neither phthalic acid, catechol and cis, cis-muconic acid metabolites were detected in culture extracts. Several enzymes （manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase） produced by ArmiUaria sp. F022 were detected during the incubation.

关 键 词：Armillaria sp. F022 dioxygenase enzymes ligninolytic enzymes naphthalene metabolites 1 4-naphthaquinone 

分 类 号：X172[环境科学与工程—环境科学]