空肠弯曲菌Lulei株WaaF基因克隆与序列分析  

Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei

作　　者：邢丛丛[1] 徐飞[1] 白欣立[2] 付金生[1] 李文倩 卢晓卫[1] 马海阳[1] 李震中[1] 

机构地区：[1]河北医科大学第二医院神经内科河北省神经病学重点实验室,石家庄050000 [2]河北医科大学第二医院儿科河北省神经病学重点实验室,石家庄050000

出　　处：《中国现代神经疾病杂志》2012年第2期158-160,共3页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：国家自然科学基金资助项目(项目编号:81072481);国家高技术研究发展计划(“863”计划)项目(项目编号:2006AA02A237)~~

摘　　要：目的克隆空肠弯曲菌Lulei株WaaF基因,并分析其遗传进化关系。方法聚合酶链反应扩增空肠弯曲菌Lulei株WaaF基因,构建pGEM-T-WaaF质粒,选择正确克隆质粒测序。自美国国家生物技术信息中心Nucleotide数据库下载5株吉兰-巴雷综合征相关菌株WaaF序列,1株非吉兰-巴雷综合征相关菌株WaaF序列,利用DNAstar v7.1软件建立进化树,分析不同菌株间WaaF基因遗传关系。结果完成了空肠弯曲菌Lulei株WaaF基因的克隆,发现WaaF基因在吉兰-巴雷综合征相关菌株间存在聚类现象。结论空肠弯曲菌Lulei株WaaF基因是807bp的片段,与Lichang株遗传进化关系最近。Objective To clone the WaaF gene of Campylobacter jejuni, and analyse its relationship with WaaF genetic evolution. Methods Amplified WaaF gene of Campylobacter jejuni Lulei by PCR, and constructed pGEM-T-WaaF cloning plasmid. Downloaded five WaaF associated with Guillain- Barre syndrome （GBS） and one WaaF not associated with GBS, and then constructed phylogcnetic tree. Results pGEM- T- WaaF cloning plasmid was constructed successfully. WaaF presented cluster phenomenon in Campylobaeter jejuni associated with GBS. Conclusion WaaF gene of Campylobacter jejuni Lulei is the fragment of 807 bp, and has the nearest relationship with the genetic evolution of Lichang.

关 键 词：弯曲杆菌 空肠 基因 序列分析 DNA 进化 遗传学 

分 类 号：R378[医药卫生—病原生物学]