HIF-1α、Tiam1表达对食管癌侵袭、淋巴结转移以及放疗的影响  

Association of HIF-1α and Tiam1 expression with tumor invasion, lymph node metastasis, and prognosis in patients with esophageal carcinoma

作　　者：范小玲[1] 刘文其[1] 毛艳[1] 康敏[1] 

机构地区：[1]广西医科大学第一附属医院放疗科,广西壮族自治区南宁市530007

出　　处：《世界华人消化杂志》2012年第7期590-595,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨食管鳞癌组织中的乏氧诱导因子-1α(HIF-1α)、T淋巴瘤侵袭转移诱导因子1(Tiam1)表达对食管癌侵袭性、淋巴结转移以及放疗疗效的影响.方法:应用免疫组织化学染色法(SP)检测HIF-1α、Tiam1在33例食管癌组织、15例正常食管黏膜的表达水平,并分析其与食管癌临床病理因素之间的关系,以及对食管癌放疗疗效和预后的影响.结果:食管癌组织HIF-1α及Tiam1蛋白阳性表达率分别是66.7%和72.7%,均明显高于正常组织(阳性表达率为6.7%,P<0.05);HIF-1α蛋白的表达程度与N分期有显著正相关性(P<0.05),Tiam1蛋白表达程度与临床分期及N分期有显著正相关性(P<0.05),而两者均与年龄、性别、病变长度、组织学分级、T分期无明显相关性;HIF-1α蛋白表达阳性组和阴性组放疗后近期完全缓解率及中位生存期分别是9.1%,9mo和63.6%,25mo,两者比较有统计学意义(P<0.05),HIF-1α蛋白阳性组与阴性组放疗后1、2、3年生存率分别为40.9%、13.6%、6.8%和90.9%、54.5%、36.4%,log-rank检验他们之间生存曲线有显著性差异(P<0.01);经相关分析,HIF-1α及Tiam1蛋白具有一定的相关性(r=0.433,P<0.05).结论:HIF-1α及Tiam1蛋白过表达对食管癌的淋巴结转移有促进作用;HIF-1α过表达对食管癌放疗有抗拒作用,因此影响了放疗的疗效.AIM： To investigate the association of expression of hypoxia inducible factor-1 alpha （HIF-1α） and T-cell lymphoma invasion and metastasis factor1 （Tiam1） with tumor invasion, lymph node metastasis, and prognosis in patients with esophageal carcinoma. METHODS： The protein expression of HIF-1α and Tiam1 was detected by immunohistochemistry in 33 esophageal carcinoma specimens and 15 normal esophageal mucosal specimens. RESULTS： The positive rates of HIF-1 and Tiam1 protein expression in esophageal carcinoma were significantly higher than those in normal esophageal mucosa （66.7% vs 6.7%, 72.7% vs 6.7%, both P 0.05）. High expression of HIF-1 was positively correlated with N stage （P 0.05）, while high expression of Tiam1 was positively correlated with TNM stage and N stage （both P 0.05）. The expression of HIF-1 and Tiam1 did not correlate with age, gender, tumor length or T stage. The complete effective rate and mean survival period after radiotherapy were significantly lower in HIF-1 -positive patients than in HIF1α-negative ones （9.1% vs 63.6%, 9 months vs 25 months, both P 0.05）. The 1-, 2-, and 3-year survival rates were also significantly lower in the HIF-1 -positive group than in the HIF-1α negative group （40.9% vs 90.9%, 13.6% vs 54.5%, 6.8% vs 36.4%）, and log-rank test suggested that the difference was significant （P = 0.007）. The expression of HIF-1α protein was significantly correlated with that of Tiam1 protein in esophageal carcinoma （r = 0.433, P = 0.012）. CONCLUSION： High expression of HIF-1α and Tiam1 proteins is associated with lymph node metastasis in patients with esophageal carcinoma. Overexpression of HIF-1α may be linked to resistance to radiotherapy in patients with esophageal carcinoma and may affect the efficacy of radiotherapy.

关 键 词：食管肿瘤 乏氧诱导因子-1Α T淋巴瘤侵袭转移诱导因子1 放射疗法 

分 类 号：R735.1[医药卫生—肿瘤]