帕瑞昔布对人胰腺癌细胞株BxPC-3、AsPC-1增殖凋亡的影响及其机制  

Parecoxib inhibits cell proliferation and induces apoptosis in human pancreatic cancer cell lines BxPC-3 and AsPC-1

作　　者：李鹏[1] 刘江伟[2] 许永华[3] 朱淑萍[3] 郭飞[2] 董翔[3] 

机构地区：[1]新疆石河子大学医学院,新疆维吾尔自治区石河子市832000 [2]中国人民解放军兰州军区乌鲁木齐总医院肝胆外科,新疆维吾尔自治区乌鲁木齐市830000 [3]中国人民解放军兰州军区乌鲁木齐总医院,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《世界华人消化杂志》2012年第8期675-679,共5页World Chinese Journal of Digestology

基　　金：中国博士后基金资助项目;No.20100481517~~

摘　　要：目的:研究帕瑞昔布(parecoxib,PCB)对人胰腺癌细胞株BxPC-3和AsPC-1的增殖、凋亡及其可能的分子机制.方法:BxPC-3、AsPC-1细胞用不同浓度PCB的培养液孵育后,利用MTT法测定细胞活性,计算IC50值,TUNEL法检测处理后细胞的凋亡情况,RT-PCR验证相关蛋白的变化表达.结果:PCB对两种细胞生长呈时间和计量依赖性抑制;PCB处理后BxPC-3、AsPC-1两种细胞IC50值为:400.98μmol/L±10.78μmol/L、256.3μmol/L±2.98μmol/L;TUNEL法检测证明凋亡率增加;RT-PCR显示COX-2表达明显降低.结论:PCB可以抑制胰腺癌细胞增殖,并诱导其凋亡生长,其可能机制是通过抑制COX-2表达来实现的.AIM： To investigate the effect of parecoxib on cell proliferation and apoptosis in human pancreatic cancer cell lines BxPC-3 and AsPC-1 and to explore possible mechanisms involved. METHODS： After BxPC-3 and AsPC-1 cells were incubated with different concentrations of parecoxib, cell viability was measured by MTT assay to calculate the half maximal inhibitory concentration （IC50）; cell apoptosis was evalu- ated by TUNEL assay; and the expression of COX-2 was detected RT-PCR. RESULTS： Cell viability was apparently inhibited by parecoxib in both cell types, and the inhibitory effect was time- and dose-dependent. The IC50 values in the two cell lines were 400.98 umol/L ± 10.78 umol/L and 256.3 umol/L ±2.98 umol/L, respectively. Treatment with parecoxib increased apoptosis rate and down-regulated COX-2 expression in both cell lines. CONCLUSION： Parecoxib potently inhibits cell proliferation and induces apoptosis in human pancreatic cancer cell lines BxPC-3 and AsPC-1 possibly by suppressing the expression of COX-2.

关 键 词：胰腺癌 帕瑞昔布 增殖 凋亡 COX-2 

分 类 号：R735.9[医药卫生—肿瘤]