PRL-3基因对结直肠癌细胞增殖能力的影响  

Role of the PRL-3 gene in the proliferation of human colon cancer SW480 cells

作　　者：柳玉红[1] 温寿青 陈蕾[1] 邱立[1] 汪春福[1] 曹亚平[1] 邹桂华[1] 

机构地区：[1]深圳市宝安区人民医院病理科,广东省深圳市518101

出　　处：《世界华人消化杂志》2012年第8期680-684,共5页World Chinese Journal of Digestology

基　　金：深圳市宝安区科学技术局社会公益科研基金资助项目;No.2009316;国家自然科学基金资助项目;No.81001110~~

摘　　要：目的:探讨PRL-3的过表达或敲低对结直肠癌细胞增殖能力的影响.方法:利用MTT法、平板克隆形成实验检测PRL-3对细胞体外增殖的影响;应用流式细胞术检测PRL-3对细胞周期的影响.结果:应用MTT法,检测PRL-3对SW480/EGFP、SW480-EGFP-PRL-3、SW480/EGFP/Mock及SW480-PRL-3-KD1细胞体外增殖能力的影响,经析因方差分析,4组差异具有显著性(F=23.463,P=0.000);不同时间点对细胞体外增殖的影响差异具有显著性(F=71.515,P=0.000);各组细胞与各时间组两因素交互效应显著(F=2.128,P=0.008);除第1天外,其他各时间点细胞组间的细胞增殖差异具有显著性.经LSD法多重比较,结果表明,与SW480/EGFP/Mock和SW480/EGFP细胞相比,SW480-EGFP-PRL-3细胞的增殖速度加快,而SW480-PRL-3-KD1细胞的增殖速度减慢.平板克隆形成实验显示SW480-EGFP-PRL-3细胞克隆形成能力明显增强,而SW480-PRL-3-KD1细胞克隆形成能力显著下降,差异具有显著的统计学意义(F=44.411,P=0.000).结论:PRL-3基因可促进结直肠癌细胞的增殖.AIM： To establish human colon cancer SW480 cell lines in which the phosphatase of regenerating liver-3 （PRL-3） gene is stably overexpressed or knocked down to study the role of this gene in regulating the biological behaviors of SW480 cells. METHODS： The impact of PRL-3 overexpression and knockdown on cell proliferation was assessed by MTT assay, colony formation assay and flow cytometry in vitro. RESULTS： Knockdown of the PRL gene significantly reduced the proliferation of SW480 cells when compared to control cells. In addition, knockdown of the PRL gene significantly impaired the ability of SW480 cells to form colonies compared to control cells. CONCLUSION： The PRL-3 gene plays an important role in the proliferation of human colon cancer SW480 cells.

关 键 词：结直肠癌 PRL-3 肿瘤增殖 

分 类 号：R735.3[医药卫生—肿瘤]