ANXA2和ANXA4在胃腺癌中的表达及临床意义  

Clinical signif icance of expression of ANXA2 and ANXA4 in gastric adenocarcinoma

作　　者：张志强[1] 聂永梅[2] 李秀娟[3] 夏宇[1] 胡翠梨[1] 苏丽萍[4] 李建洲[4] 宋娜[4] 蒋海[4] 张洁[4] 

机构地区：[1]新疆医科大学第一附属医院消化科,新疆维吾尔自治区乌鲁木齐市830011 [2]新疆医科大学基础医学院生理学教研室,新疆维吾尔自治区乌鲁木齐市830011 [3]新疆医科大学基础医学院病理生理学教研室,新疆维吾尔自治区乌鲁木齐市830011 [4]新疆医科大学基础医学院,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《世界华人消化杂志》2012年第11期930-935,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81001101;新疆维吾尔自治区自然科学基金资助项目;No.2010211B20;新疆维吾尔自治区高校科研计划青年教师科研启动基金资助项目;No.XJEDU2010S24;自治区卫生厅青年科技人才专项科研基金资助项目;No.2009Y06~~

摘　　要：目的:研究膜联蛋白A2(AnnexinA2,ANXA2)、膜联蛋白A4(AnnexinA4,ANXA4)在人体胃腺癌(gastric adenocarcinoma cells,GAC)的表达,探讨其表达与GAC生物学行为的关系及两种蛋白的相关性.方法:免疫组织化学SP法检测组织芯片中75对配对的GAC和其相应癌旁组织ANXA2和ANXA4的表达.结果:在GAC和癌旁组织中ANXA2、ANXA4的表达分别为:33.1%(25)vs1.3%(1)、68.0%(51)vs25.5%(13),二者在GAC的表达显著高于癌旁组织(x2=24.448,P=0.000;x2=39.353,P=0.000);ANXA2和ANXA4在GAC表达呈正相关(r=0.335,P=0.003);两种蛋白的表达与GAC的浸润深度、淋巴结转移、TNM分期和分化程度有关(P<0.05),与年龄、性别和远处转移无关(P>0.05);肿瘤大小与ANXA2表达有关(P<0.01),与ANXA4表达无关(P>0.05).结论:ANXA2和ANXA4的表达上调可能共同参与了GAC的发生、发展、浸润和转移,可作为临床预测GAC预后的标志物和治疗靶点.AIM：To investigate the expression of annexin A2（ANXA2） and annexin A4（ANXA4） in human gastric adenocarcinoma（GAC） and to explore the relationship between their expression and biological behaviors of GAC.METHODS：The expression of ANXA2 and ANXA4 was detected by immunohistochemistry in a tissue microarray containing 75 pairs of GAC and matched tumor-adjacent tissue specimens.RESULTS：The positive rates of ANXA2 and ANXA4 expression in GAC were significantly higher than those in tumor-adjacent tissue（33.1% vs 1.3%,68.0% vs 25.5%,both P 0.05）.The expression of ANXA2 was positively correlated with that of ANXA4 in GAC（r = 0.335,P = 0.003）.The expression of ANXA2 and ANXA4 was closely associated with invasion depth,lymph node metastasis,TNM stage and differentiation grade in GAC（all P 0.05）,but not with sex,age or distant metastasis（all P 0.05）.Tumor size had a correlation with the expression of ANXA2（P 0.05）,but not with that of ANXA4（P 0.05）.CONCLUSION：Overexpression of ANXA2 and ANXA4 may play a synergistic role in the carcinogenesis,invasion and metastasis of GAC.ANXA2 and ANXA4 may be used as targets for GAC therapy and markers for prognostic prediction.

关 键 词：胃腺癌 膜联蛋白A2 膜联蛋白A4 组织芯片 

分 类 号：R735.2[医药卫生—肿瘤]