人脑肿瘤猴病毒40的感染状况及来源研究  

A study of simian virus 40 infection and its origin in human brain tumors

作　　者：步星耀[1] 章翔[2] 张旭[3] 易声禹[2] 

机构地区：[1]河南省人民医院神经外科,郑州450003 [2]第四军医大学西京医院全军神经疾病研究所 [3]第四军医大学全军神经科学研究所

出　　处：《中华流行病学杂志》2000年第1期19-21,共3页Chinese Journal of Epidemiology

基　　金：中国博士后科学基金! (编号中博基 - 2 3)

摘　　要：目的　探讨人脑肿瘤猴病毒40(SV40)的感染状况及来源。方法　采用聚合酶链反应(PCR)和斑点杂交方法,检测人脑肿瘤组织516份,健康人外周血80份,精液50份,人胚胎组织100份,正常人脑组织30份以及SHG44和BT325两株人脑胶质瘤细胞系中SV40的DNA序列。结果人脑肿瘤组织SV40DNA阳性率为36.4%(188/516);健康人外周血16.3%(13/80);精液22.0%(11/50);人胚胎组织8.0%(8/100);正常人脑组织6.7%(2/30)。SHG44及BT325两株细胞中也分别检测出SV40的DNA序列。人脑肿瘤组织、健康人外周血及精液与健康人脑组织及人胚胎组织阳性率相比,差异有显著性(P<0.05)。结论　①人脑肿瘤有较高的SV40感染率;②SV40与人脑肿瘤的发生有密切关系;Objective To study the status of simian virus 40 (SV40) infection and its origin in human brain tumors. Methods Polymerase chain reaction (PCR) and Dot blot hybridization were used to detect SV40 DNA sequences in 516 human brain tumor tissues, 80 peripheral blood cells and 50 sperm fluids from healthy individuals, 100 human embryo tissues from artificial abortion, 30 normal brain tissues and two human glioma cell lines: SHG44 and BT325. Results SV40 DNA sequences were found in 36.4 % of human brain tumors (188/516), 16.3 % of healthy peripheral blood cells (13/80), 22.0 % of healthy semen (11/50), 8.0 % of human embryo tissues (8/100) and 6.7 % of normal brain tissues (2/30). SV40 DNA sequences were also detected in SHG44 and BT325 cell lines. The positive rates of SV40 DNA in human brain tumors, peripheral blood cells and semen from healthy donors were significantly higher than those in human normal brain and embryo tissues ( P < 0.05 ). Conclusions ①SV40 has a higher infection rate in human brain tumors,②SV40 is closely related to the etiopathogenesis of human brain tumors,③the ways of SV40 spread may due to both horizontal and diaplacental infections in human population.

关 键 词：脑肿瘤 猴病毒40感染 聚合酶链反应 斑点杂交 

分 类 号：R73-31[医药卫生—肿瘤]