绿脓杆菌对角膜基质细胞介导的胶原降解作用的实验研究  

Effect on keratocyte mediated collagen degradation by Pseudomonas aeruginosa

作　　者：郝继龙[1] 陆瑛[1] 贾卉[1] 刘景祥[1] 徐锦春[1] 张瑞雪[1] 

机构地区：[1]白求恩医科大学第三临床学院眼科,长春130031

出　　处：《中华眼科杂志》2000年第1期43-45,共3页Chinese Journal of Ophthalmology

摘　　要：目的　探讨绿脓杆菌性角膜炎组织破坏机制 ,为临床治疗提供理论依据。方法　Ⅰ型胶原凝胶 ,混悬及不混悬角膜基质细胞 ,与绿脓杆菌培养液共同培养 2 4h ,超滤去除天然胶原纤维 ,超滤液经过水解 ,分光光度计测定其羟脯氨酸含量。同时检测基质金属蛋白酶 (matrixmetalloproteinase ,MMP)抑制剂Galardin对胶原降解作用的影响。结果　绿脓杆菌培养液可直接降解Ⅰ型胶原 ,如同时存在角膜基质细胞此作用增强。无论角膜基质细胞存在与否 ,Galardin可明显降低绿脓杆菌所致的胶原降解作用。结论　绿脓杆菌培养液直接降解Ⅰ型胶原凝胶 ,同时增强角膜基质细胞介导的胶原降解作用 ,为绿脓杆菌性角膜炎治疗提供广阔应用前景。Objective To study the pathogenesis of cornea melting (ulceration) by pseudomona (P) aeruginosa for instruction of clinical treatment. Methods Type Ⅰ collagen gels with or without suspended keratocytes were incubated for 24 hours under medium containing sterile P. aeruginosa culture broth. Native collagen fibrils were removed from the media by ultrafiltration. The ultrafiltrates were then hydrolyzed, and the amount of hydroxyproline was measured spectrophotometrically. The effect of a synthetic matrix metalloproteinase(MMP) inhibitor, Galardin, on collagen degradation was also examined. Results P. aeruginosa broth induced type Ⅰ collagen gel degradation directly. In the presence of keratocytes, degradation by P. aeruginosa broth was enhanced. Galardin significantly reduced the amount of collagen degraded by P. aeruginosa culture broth, no matter keratocytes were present or not. Conclusion P. aeruginosa culture broth directly degrades type Ⅰ collagen and also increases keratocyte mediated collagen degradation. The result is helpful to the clinical treatment of cornea melting caused by P. aeruginosa, and the mechanism should be further studied.

关 键 词：绿脓肝菌 胶原 角膜炎 角膜基质细胞 

分 类 号：R772.21[医药卫生—眼科]