RT-PCR方法诊断大肠癌淋巴结微转移  

Detection of lymph node micrometastases in colorectal carcinoma by reverse transcriptase-polymerase chain reaction.

作　　者：万远廉[1] 潘义生[1] 刘玉村[1] 郭源[1] 魏群[1] 

机构地区：[1]北京医科大学第一医院外科,100034

出　　处：《中国实用外科杂志》2000年第1期47-48,共2页Chinese Journal of Practical Surgery

摘　　要：目的　探讨提高大肠癌淋巴结微转移诊断率的敏感方法。方法　用逆转录聚合酶链式反应 (RT -PCR)检测基质金属蛋白酶 - 7(MMP - 7)mRNA在 38例大肠癌和 2 5个淋巴结中的表达 ,对部分淋巴结再次行组织学检查。结果　 97 4% (37/ 38)的大肠癌中MMP - 7mRNA表达阳性 ,而正常粘膜几乎不表达。传统组织学检查发现癌转移的 13个淋巴结MMP - 7mRNA表达均为阳性 ;未见转移的 12个淋巴结MMP - 7mRNA表达6个阴性、6个阳性 ,再次行组织学检查于 6个MMP - 7阳性者中发现 3个有微转移。结论　RT -PCR方法检测淋巴结中MMP - 7mRNA的表达是诊断大肠癌淋巴结微转移的敏感方法 ,可以作为传统组织学检查的补充。Objective\ To investigate a sensitive method for detecting lymph node micrometastases in colorectal carcinoma.Methods\ RT-PCR technique was used to examine the expression of MMP-7 mRNA in colorectal carcinomas and lymph nodes.Histological re-examination was performed on some of the lymph nodes.Results\ MMP-7 mRNA was observed in 97 4%(37/38) colorectal carcinomas,while it was undetectable in almost all the normal mucosas.All 13 histologically-positive lymph nodes were positive for MMP-7,while of 12 histologically-negative lymph nodes,6 were positive and the other 6 were negative.When histological re-examination was performed on histologically-negative lymph nodes,micrometastases were detected in 3“MMP-7 positive”lymph nodes.Conclusion\ RT-PCR assay for MMP-7 mRNA is a sensitive method for detecting lymph node micrometastases in patients with colorectal carcinoma,and may complement conventional histopathology.

关 键 词：大肠肿瘤 肿瘤转移 淋巴结微转移 RT-PCR 

分 类 号：R735.340.4[医药卫生—肿瘤] R73-37[医药卫生—临床医学]