膀胱癌中bcl-2和p16基因的表达与预后的关系  

Expression of bcl-2 and p16 in transitional cell carcinoma of urinary bladder

作　　者：冷江涌[1] 张亚正[1] 姚旭东[1] 温凯胜[1] 

机构地区：[1]内蒙古医学院第一附属医院泌尿外科,呼和浩特010050

出　　处：《中华外科杂志》2000年第1期40-43,共4页Chinese Journal of Surgery

摘　　要：目的　探讨bcl 2和p16基因蛋白在膀胱癌的表达与预后的关系。　方法　采用SABC法对 5 1例膀胱癌组织和 5例正常膀胱粘膜行bcl 2和p16基因表达的检测。　结果　bcl 2在膀胱癌的阳性表达率为 80 2 % (42 / 5 1)。生存组和死亡组之间比较差异有显著性意义 (P <0 0 5 )。p16在膀胱癌的阳性表达率为 5 0 9% (2 6 / 5 1)。 5例正常膀胱粘膜均阳性 ,两者比较差异有显著性意义 (P <0 0 5 )。在病理分级、临床分期和预后的总样本率中比较差异有显著性意义 (P <0 0 5 ) ;即随病理分级临床分期的上升和预后的不良 ,p16的阳性表达下降。bcl 2阳性而p16阴性 (2 1例 )的分布随病理分级、临床分期的上升和预后不良而上升 (P <0 0 5 )。bcl 2和p16都阳性 (2 2例 )的分布 ,随病理分级、临床分期升高和预后不良而下降 (P <0 0 5 )。　结论　原癌基因bcl 2过度表达和抑癌基因p16缺失突变与膀胱癌的发生发展及预后有密切关系。可作为评估膀胱癌恶性程度及预后的指标。Objective[WT5”BZ] To study the relationship between the expression of proto oncogene bcl 2 and MTS1/p16 in transitional cell carcinoma of bladder and prognosis. [WT5”HZ]Methods[WT5”BZ] Immunohistochemical SABC method was used to detect the expression of proto oncogene bcl 2 and MTS1/p16 in 51 paraffin embedded primary transitional cell carcinoma of bladder specimens and 5 normal bladder mucosa specimens. [WT5”HZ]Results[WT5”BZ] The positive expression of bcl 2 was seen in 80 2%(42/51) tumors. There was statistically significant correlation between prognosis and the positive expression of bcl 2. The positive expression rate was higher in tumors with poorer prognosis than in those with better prognosis ( P <0 05).The expression of p16 protein was positive in 50 9%(26 of 51) in tumors and negative in all 5 normal bladder mucosae. The positive expression rate of p16 descended with increasing grade, stage and poorer prognosis. Clinicopathological characteristics and prognosis were related to the expression of p16 ( P <0 05).The positive expression of bcl 2 versus negtive expression of p16 were seen in 20 of 51 of tumors, with distributing gradeⅠ3/11, gradeⅡ 9/26, grade Ⅲ 8/14, Tis～T 1 6/28, T 2 10/17, T 3 4/6, A group 5/20, B group 4/16, and C group 11/15, respectively. Both bcl 2 and p16 were positively expressed in 22 of 51 of tumors. Their positive expressions were higher in tumors with lower grade, stage and better prognosis than in those with worse clinicopathological characteristics and poorer prognosis ( P <0 05). [WT5”HZ]Conclusions[WT5”BZ] Over expression of bcl 2 appears to be common in bladder cancer; over expression of proto oncogene bcl 2 and inactivation of the MTS1/p16 gene are likely to be contributing factors for primary bladder cancer; and they can be the prognostic indicators for transitional cell carcinoma of urinary bladder. [WT5”HZ]

关 键 词：膀胱肿瘤 基因表达 预后 BCL-2基因 P16基因 

分 类 号：R737.14[医药卫生—肿瘤]