经右锁骨下动脉、右房插管兔体外循环及选择性脑灌注模型的建立  

Establishment of rabbit model of cardiopulmonary bypass and selective cerebral perfusion via right subclavian artery and right atrium cannulation

作　　者：杨九光[1] 龙村[1] 姚忠喜[2] 刘旭 郑红[1] 黑飞龙[1] 史世勇[1] 温复兴[1] 李景文 刘晋萍[1] 冯正义[1] 

机构地区：[1]阜外心血管病医院麻醉体外循环研究室,北京100037 [2]兰州医学院第一附属医院心血管外科

出　　处：《中华实验外科杂志》2000年第1期77-78,共2页Chinese Journal of Experimental Surgery

基　　金：卫生部科研基金资助!( 96 1 0 43 )

摘　　要：目的　建立体外循环 (cardiopulmonarybypass,CPB)及深低温停循环 (deephypothermiacirculatoryarrest,DHCA)时选择性脑灌注 (selectivecerebralperfusion,SCP)兔模型。方法　选用大耳白兔2 9只 ,经右锁骨下动脉及右房插管建立CPB ,鼻温 2 0℃时停循环或SCP 80min,监测血流动力学指标及血气变化。结果　在CPB中动物血流动力学稳定 ,心脏自动复跳率较高 ,为 75 .9%。自制微型氧合器氧合性能完全符合满意CPB标准。结论　经右锁骨下动脉、右房插管兔体外循环及选择性脑灌注建立的模型安全、稳定、简单、实用 ,是进行深低温停循环时选择性灌注脑保护研究的较理想模型。Objective To establish a rabbit model of the cardiopulmonary bypass (CPB) and the selective cerebral perfusion (SCP) during deep hypothermia circulatory arrest (DHCA). Methods Twenty nine rabbits were assigned to undergo 80 min of DHCA or SCP at an nasopharynx temperature of 20℃. CPB was established via right subclavian artery and right atrium cannulation. The hemodynamics and the blood gas parameters were observed during the experiment. Results The hemodynamical parameters were steady during CPB, and the rabbits also had a high automatic recovery rate of heart beat. The oxygenation property of the self made miniature oxygenator was sufficient to fit the standard of satisfactory CPB. Conclusion The rabbit CPB and SCP model established via right subclavian artery and right atrium cannulation was safe, reliable, steady and utility, and is an ideal model for cerebral protection experimental research of SCP during DHCA.

关 键 词：体外循环 脑保护 深低温停循环 脑缺血 兔 

分 类 号：R-332[医药卫生]