干扰素α-2b与实验性滤过性手术  

Glaucoma filtering surgery with interferon alpha 2b in white rabbits

作　　者：张秀兰[1] 彭大伟[1] 曾淑君[1] 郑湖玲[1] 梁美玉 

机构地区：[1]中山医科大学中山眼科中心,510060

出　　处：《中国实用眼科杂志》2000年第1期26-28,共3页Chinese Journal of Practical Ophthalmology

基　　金：广东省自然科学基金

摘　　要：为探讨干扰素α－２ｂ（ｉｎｔｅｒｆｅｒｏｎ－ａｌｐｈａ２ｂ，ＩＦＮα－２ｂ）在不同给药方法及不同剂量下对兔眼抗青光眼术后滤过泡的影响，选取新西兰纯种白兔２０只，全部行标准统一的巩膜咬切术，采用湿棉片敷贴给药和结膜下注射的方法，与对照组相比，观察术后的疗效，包括滤过泡存留情况、滤过区组织学检查、成纤维细胞计数和术后并发症。结果：湿棉片敷贴５×１０５ＩＵ／ｍｌ或１×１０６ＩＵ／ｍｌＩＦＮα－２ｂ５分钟，与对照组相比，疗效均无明显差别；结膜下注射５×１０５ＩＵ或１×１０５ＩＵ能有效减少滤过区疤痕组织增生，且５×１０５ＩＵ效果比１×１０５ＩＵ好；实验条件下，未引起明显的眼部并发症。结论：ＩＦＮα－２ｂ能有效抑制术后滤过道疤痕形成、提高滤过泡存活率；采用类似丝裂霉素Ｃ（ｍｉｔｏｍｙｃｉｎｃ，ＭＭＣ）给药方法，对防止滤过泡疤痕化无明显作用；结膜下注射给药可能更适合ＩＦＮα－２ｂ，每次注射剂量以５×１０５ＩＵ效果较好。To evaluate the effect of interferon alpha 2b(IFNα 2b)on rabbit filtration surgery,20 rabbits(40 eyes)were be availabled to perform sclerectomy.Topical application of IFN α 2b were be taken via a sponge and subconjunctival injection.The results of exprimental and control group were be comparied by filtering blebs formation,histologic examinations including fibroblast cell counts in filtering area and postcomplications.Results No significant suppression effect was seen on fibrotic scar formation by topical used 5×10 5IU or 1×10 6IU/ml IFNα 2b for 5 minutes via a sponge which way was similar with Mitomycin C(MMC)whereas subconjunctivally adminstered 5×10 5IU or 1×10 6IU were revealed successful results.The result of 5×10 6IU dosage group seemed to be better than that of 1×10 5IU group by indicating more filting blebs formation,less scar tissue crowding in filtration area and fibroblasts proliferation under the light microscope.In addition,no sign of inflammation was observed in all rabbits.Conclusions IFNα 2b could indeed reduce the scar and improve filtering bleb formation with no significant side effects or complications.Dosage 5×10 5IU of IFNα 2b could be considered to be used clinically.

关 键 词：青光眼 滤过性手术 干扰素Α-2B 

分 类 号：R775.05[医药卫生—眼科]