洛铂对肺癌HTB-56、HTB-56/DDP细胞株生长抑制作用的研究  

Research on growth inhibitoty of HTB-56 cells and HTB-56/DDP cells in lung cancer treated with lobaplatin

作　　者：高文斌[1] 邓蓉[1] 韩佩妍[1] 黄剑辉[1] 李花妮[1] 马建增[1] 郑真真[1] 方今女[2] 

机构地区：[1]大连大学附属中山医院肿瘤内科,辽宁大连116001 [2]延边大学医学部病理及病理生理学教研室,吉林延吉133002

出　　处：《中华肺部疾病杂志（电子版）》2012年第2期33-35,共3页Chinese Journal of Lung Diseases(Electronic Edition)

基　　金：CSCO-金港榄香烯肿瘤学研究生奖学助学基金(J-2010-008)

摘　　要：目的探讨不同浓度洛铂(LBP)对肺癌HTB-56、HTB-56/DDP细胞株生长抑制作用的影响,及不同药物浓度对细胞影响之间的关系。方法选择人肺癌HTB-56及其耐DDP细胞株HTB-56/DDP为研究对象,用MTT比色法检测不同浓度LBP作用于腺癌细胞株不同时间的OD值(optical density,光密度),比较药物剂量与其生长抑制效应之间的关系。结果 LBP能够抑制肺癌细胞株HTB-56及其耐DDP细胞株HTB-56/DDP的生长,并呈浓度与时间的依赖关系,随着浓度的升高和时间的延长对两种细胞的抑制率也随之增大。结论 LBP能够显著的抑制HTB-56及其耐DDP细胞株HTB-56/DDP的细胞增殖,并不与DDP交叉耐药。随着小剂量LBP作用时间的延长,细胞的生长抑制呈现浓度与时间的依赖。Objective To discuss the effect on growth inhibitoty of lung adenocarcinoma cell treated by lobaplatin （LBP） in different doses, and the relationship between the effects and diverse doses. Methods Human lung adenocarcinoma cell lines HTB-56 and HTB-56/DDT cell lines were treated with different doses and lasting time of LBP to measure the OD （optical density） at different time by MTT, compare the relationships between dose of LBP and the growth control effect. Results LBP can control growth of lung adenocarcinoma cell lines HTB-56 and HTB-56/DDT cell lines which relate to dose and enduring time, control rate was upgrading with the increasing of the dose of LBP and time lasting. Conclusions LBP can control the growth of lung adenocarcinoma cell lines HTB-56 and HTB-56/DDT cell lines. Make sure LBP isn’t cross resistance with DDP. Growth control of lung adenocarcinoma cell related to the dose and enduring time when the continuous low dose of LBP treatment was lasting.

关 键 词：支气管肺癌 HTB-56、HTB-56/DDP细胞株 洛铂 MTT 

分 类 号：R734.2[医药卫生—肿瘤]