Quantification of choline concentration following liver cell apoptosis using ~1H magnetic resonance spectroscopy  

作　　者：Zhi-Wei Shen Zhen Cao Ke-Zeng You Zhong-Xian Yang Ye-Yu Xiao Xiao-Fang Cheng Yao-Wen Chen Ren-Hua Wu 

机构地区：[1]Department of Medical Imaging, The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong Province, China [2]Central Laboratory, Shantou University, Shantou 515041, Guangdong Province, China [3]Provincial Key Laboratory of Medical Molecular Imaging, Shantou 515041, Guangdong Province, China

出　　处：《World Journal of Gastroenterology》2012年第10期1130-1136,共7页世界胃肠病学杂志（英文版）

基　　金：Supported by Grants from Medical Scientific Research Foundation of Guangdong Province, China, No. B2008128;National Natural Science Foundation of China, No. 30930027 and No. 60971075, in part

摘　　要：AIM: To evaluate the feasibility of quantifying liver choline concentrations in both normal and apoptotic rabbit livers in vivo, using 1H magnetic resonance spectroscopy (1H-MRS). METHODS: 1H-MRS was performed in 18 rabbits using a 1.5T GE MR system with an eight-channel head/neck receiving coil. Fifteen rabbits were injected with sodium selenite at a dose of 10 μmol/kg to induce the liver cell apoptosis. Point-resolved spectroscopy sequencelocalized spectra were obtained from 10 livers once before and once 24 h after sodium selenite injection in vivo. T1 and T2 relaxation time of water and choline was measured separately in the livers of three healthy rabbits and three selenite-treated rabbits. Hematoxylin and eosin and dUTP-biotin nick end labeling (TUNEL) staining was used to detect and confirm apoptosis. Choline peak areas were measured relative to unsuppressed water using LCModel. Relaxation attenuation was corrected using the average of T1 and T2 relaxation time. The choline concentration was quantified using a formula, which was tested by a phantom with a known concentration. RESULTS: Apoptosis of hepatic cells was confirmed by TUNEL assay. In phantom experiment, the choline concentration (3.01 mmol/L), measured by 1H-MRS, was in good agreement with the actual concentration (3 mmol/L). The average T1 and T2 relaxation time of choline was 612 ± 15 ms and 74 ± 4 ms in the control group and 670 ± 27 ms and 78 ± 5 ms in apoptotic livers in vivo, respectively. Choline was quantified in 10 rabbits, once before and once after the injection with sodium selenite. The choline concentration decreased from 14.5 ± 7.57 mmol/L before sodium selenite injection to 10.8 ± 6.58 mmol/L (mean ± SD, n = 10) after treatment (Z = -2.395, P < 0.05, two-sample paired Wilcoxon test). CONCLUSION: 1H-MRS can be used to quantify liver choline in vivo using unsuppressed water as an internal reference. Decreased liver choline concentrations are found in sodium selenite-treated rabbits undergoing liver cell apoptosis.AIM： To evaluate the feasibility of quantifying liver choline concentrations in both normal and apoptotic rabbit livers in vivo, using 1H magnetic resonance spectroscopy （1H-MRS）. METHODS： 1H-MRS was performed in 18 rabbits using a 1.5T GE MR system with an eight-channel head/neck receiving coil. Fifteen rabbits were injected with sodium selenite at a dose of 10 μmol/kg to induce the liver cell apoptosis. Point-resolved spectroscopy sequencelocalized spectra were obtained from 10 livers once before and once 24 h after sodium selenite injection in vivo. T1 and T2 relaxation time of water and choline was measured separately in the livers of three healthy rabbits and three selenite-treated rabbits. Hematoxylin and eosin and dUTP-biotin nick end labeling （TUNEL） staining was used to detect and confirm apoptosis. Choline peak areas were measured relative to unsuppressed water using LCModel. Relaxation attenuation was corrected using the average of T1 and T2 relaxation time. The choline concentration was quantified using a formula, which was tested by a phantom with a known concentration. RESULTS： Apoptosis of hepatic cells was confirmed by TUNEL assay. In phantom experiment, the choline concentration （3.01 mmol/L）, measured by 1H-MRS, was in good agreement with the actual concentration （3 mmol/L）. The average T1 and T2 relaxation time of choline was 612 ± 15 ms and 74 ± 4 ms in the control group and 670 ± 27 ms and 78 ± 5 ms in apoptotic livers in vivo, respectively. Choline was quantified in 10 rabbits, once before and once after the injection with sodium selenite. The choline concentration decreased from 14.5 ± 7.57 mmol/L before sodium selenite injection to 10.8 ± 6.58 mmol/L （mean ± SD, n = 10） after treatment （Z = -2.395, P 〈 0.05, two-sample paired Wilcoxon test）. CONCLUSION： 1H-MRS can be used to quantify liver choline in vivo using unsuppressed water as an internal reference. Decreased liver choline concentrations are found in sodium selenite-treated rabbits undergoing

关 键 词：Cell apoptosis Magnetic resonance spectroscopy Quantification CHOLINE In vivo 

分 类 号：S858.31[农业科学—临床兽医学] Q255[农业科学—兽医学]