Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers  

作　　者：Nina Kocevar Federico Odreman Alessandro Vindigni Snjezana Frkovic Grazio Radovan Komel 

机构地区：[1]Medical Centre for Molecular Biology,Institute of Biochemistry,Faculty of Medicine,University of Ljubljana [2]International Centre for Genetic Engineering and Biotechnology Trieste,AREA Science Park,Trieste 34149,Italy [3]Department of Obstetrics and Gynecology,Department of Gynecological Pathology and Cytology, University Clinical Hospital Ljubljana,Ljubljana 1000,Slovenia

出　　处：《World Journal of Gastroenterology》2012年第11期1216-1228,共13页世界胃肠病学杂志（英文版）

基　　金：Supported by Research grant,No.P1-0104-0386;the Junior Researcher Grant,No.1000-07-310086,awarded to Kocevar N,both from the Slovenian Research Agency

摘　　要：AIM： To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma.METHODS： We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting.RESULTS： We identified 30 different proteins involved in various biological processes： metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteinswere chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 （mitochondrial precursor）, plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, sep- tin-2, ubiquitin-conjugating enzyme E2 N, and transaldolase were significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border.CONCLUSION： This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis,AIM:To search or and validate di erentially expressed proteins in patients with gastric adenocarcinoma.METHODS:We used two-dimensional gel electrophoresis and mass spectrometry to search or di erentially expressed proteins in patients with gastric adenocarcinoma.A set o proteins was validated with immunoblotting.RESULTS:We identified 30 di erent proteins involved in various biological processes:metabolism,development,death,response to stress,cell cycle,cell communication,transport,and cell motility.Eight proteins were chosen or urther validation by immunoblotting.Our results show that gastrokine-1,39S ribosomal protein L12(mitochondrial precursor),plasma cell-induced resident endoplasmic reticulum protein,and glutathione S-trans erase mu 3 were signi icantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples.On the other hand,septin-2,ubiquitin-conjugating enzyme E2 N,and transaldolase were significantly overexpressed.Translationally controlled tumor protein was shown to be di erentially expressed only in patients with cancer o the gastric cardia/esophageal border.CONCLUSION:This work presents a set o possible diagnostic biomarkers,validated or the first time.It might contribute to the e orts o understanding gastric cancer carcinogenesis.

关 键 词：Two-dimensional gel electrophoresis MASSSPECTROMETRY Gastric adenocarcinoma PROTEOME Bi-omarkers 

分 类 号：R735.2[医药卫生—肿瘤]