重组人促红细胞生成素干预人源羊水干细胞向心肌前体细胞的分化  

Effect of recombinant human erythropoietin on differentiation of myocardial precusor cells from amniotic fluid stem cells of pregnancy women

作　　者：刘燕[1] 李法琦[1] 张彬[2] 骆建平[3] 

机构地区：[1]重庆医科大学附属第一医院,老年科,重庆市400016 [2]重钢总医院心内科,重庆市400080 [3]重庆医科大学附属第一医院,临床产前诊断中心,重庆市400016

出　　处：《中国组织工程研究》2012年第10期1761-1764,共4页Chinese Journal of Tissue Engineering Research

基　　金：重庆市卫生局医学科研项目(2009-2-367),课题名称:促红细胞生成素调节经典Wnt信号途径促进羊水来源诱导多能干细胞心肌定向分化的研究~~

摘　　要：背景:研究发现人源羊水干细胞可向心肌前体细胞分化。目的:观察不同浓度重组人促红细胞生成素对人源羊水干细胞向心肌前体细胞分化的影响。方法:用含0,1.0,5.0,10.0,20.0U/mL重组人促红细胞生成素的诱导培养基诱导鉴定后的人源羊水干细胞向心肌前体细胞分化,诱导24h后换为完全培养基继续培养。结果与结论:诱导分化14d,倒置显微镜下可见细胞由长梭形逐渐变短增宽,相邻的细胞间有融合现象,似类肌管样结构,以5.0U/mL重组人促红细胞生成素的诱导分化率最高;RT-PCR检测显示重组人促红细胞生成素可促进细胞Nkx-2.5和GATA-4mRNA的表达,以5.0U/mL重组人促红细胞生成素的作用最强。说明外源性重组人促红细胞生成素能剂量依赖性促进人源羊水干细胞向心肌前体细胞分化。BACKGROUND：Studies have shown that amniotic fluid stem cells of pregnancy women can differentiate into myocardial precursor cells.OBJECTIVE：To study the effect of recombinant human erythropoietin（rhEPO） at different concentrations on differentiation of myocardial precusor cells from amniotic fluid stem cells of pregnancy women.METHODS：The differentiation of myocardial precursor cells from amniotic fluid stem cells of pregnancy women was induced by adding conditional culture medium containing 0,1.0,5.0,10.0,20.0 U/mL rhEPO.After 24 hours,conditional culture medium was replaced by complete medium.RESULTS AND CONCLUSION：During 14 days of induced differentiation,under the inverted microscope,cells gradually shortened and widened from original shuttle appearance.Adjacent cells fused together,similar to myotube-like structure.The differentiation rate of myocardial precursor cells was highest after 5.0 U/mL rhEPO addition.RT-PCR results showed that rhEPO particularly at the concentration of 5.0 U/mL could promote Nkx-2.5 and GATA-4 mRNA expression.These findings suggest that exogenous rhEPO can promote the differentiation of myocardial precursor cells from amniotic fluid stem cells of pregnancy women dose-dependently.

关 键 词：重组人促红细胞生成素 人源羊水干细胞 心肌前体细胞 Nkx-2.5 GATA-4 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]