微小RNA-155在小鼠骨髓来源M1和M2巨噬细胞中表达的差异  

Diverse expression patterns of microRNA-155 between bone marrow-derived M1 and M2 macrophages

作　　者：梁艳冰[1] 王中华[1] 唐皓[1] 马中富[1] 

机构地区：[1]中山大学附属第一医院普内科,广东省广州市510080

出　　处：《中国组织工程研究》2012年第10期1866-1870,共5页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目(2009B080701070;2009B080701004);课题名称:miRNA-155在调控脓毒症小鼠SOCS1通路中作用的研究;IL-4/IL-13活化的M2巨噬细胞对脓毒症的免疫调节机制~~

摘　　要：背景:目前对微小RNA-155在巨噬细胞中的表达和功能的研究还集中在总体单核巨噬细胞水平。目的:了解小鼠骨髓来源M1和M2巨噬细胞中微小RNA-155表达的差异。方法:用100U/mL干扰素γ和5μg/L脂多糖诱导骨髓细胞来源的巨噬细胞向M1分化,用10μg/L白细胞介素4诱导出M2巨噬细胞。结果与结论:流式细胞检测显示实验诱导的M1和M2巨噬细胞纯度分别达91%和95%。RT-PCR检测显示诱导型一氧化氮合酶mRNA在M1巨噬细胞中高表达,在M2中则基本不表达;而Ⅰ型精氨酸酶、发现于炎症区域1mRNA在M2中高表达,在M1中则表达较低;炎症因子肿瘤坏死因子αmRNA在M1中的表达明显高于M2,相反白细胞介素10mRNA在M2中的表达高于M1(P<0.05)。实时荧光定量PCR检测显示M1巨噬细胞中微小RNA-155的表达量明显高于M2(P<0.05)。提示微小RNA-155可作为鉴别M1,M2巨噬细胞的标志。BACKGROUND：Previous studies of microRNA-155 expression and function in macrophage predominantly focus on total mononuclear macrophage.OBJCTIVE：To evaluate the expression difference of microRNA-155 in M1 and M2 macrophages.METHODS：Bone marrow-derived macrophages were induced to differentiate towards M1 and M2 macrophages using interferon-γ（100 U/mL） ＋ lipopolysaccharide（5 ng/mL） and interleukin-4（10 ng/mL）,respectively.RESULTS AND CONCLUSION：The differentiation proportion of M1 and M2 macrophages detected by flow cytometry was 91% and 95%,respectively.Reverse transcription PCR method showed that inducible nitric oxide synthase（iNOS） mRNA was highly expressed in M1 macrophages,but it was hardly expressed in M2 macrophages.Arginase-1 and found in inflammatory zone 1 mRNA expression was highly expressed in M2 macrophages,but the expression was low in M1 macrophages.Tumor necrosis factor α mRNA expression was significantly higher in M1 macrophages than in M2 macrophages,on the contrary,interleukin 10 mRNA expression was significantly higher in M2 macrophages than in M1 macrophages（P 0.05）.Real-time fluorescence quantitative PCR showed that microRNA-155 expression in M1 macrophages was significantly higher compared with that in M2 macrophages（P 0.05）.These findings suggest that MiRNA-155 can serve as a useful maker for differential diagnosis of M1 and M2 macrophages.

关 键 词：微小RNA-155 骨髓细胞 M1巨噬细胞 M2巨噬细胞 小鼠 基因表达 

分 类 号：R394.2[医药卫生—医学遗传学]