火炭母提取物抑菌活性的初步研究  

Preliminary Research of Antimicrobial Activity of the Extract from Polygonum Chinense Linn.

作　　者：欧阳蒲月[1,2,3] 朱翠霞[1] 陈功锡[2] 邓玲芳[2] 

机构地区：[1]广东食品药品职业学院,广东广州510520 [2]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000 [3]南药资源保护与利用工程技术开发中心,广东广州510520

出　　处：《化学与生物工程》2012年第4期37-40,44,共5页Chemistry & Bioengineering

基　　金：植物资源保护与利用湖南省高校重点实验室开放项目(JZ200906);南药资源保护与利用工程技术开发中心建设项目(GC-ZX-B0905)

摘　　要：对药用植物火炭母(Polygonum chinense Linn.)提取物的抑菌活性进行了研究。结果表明:(1)不同部位提取物中,叶和茎均含有抑菌活性物质,但叶提取物的抑菌效果更强、更明显;(2)不同溶剂提取物中,65%乙醇提取物具有最佳抑菌效果;(3)不同提取方法提取物中,超声提取物抑菌效果较索氏提取物抑菌效果强,但差异不是很大;(4)不同溶剂萃取物的抑菌实验表明,火炭母提取物中的抑菌活性物质易溶于乙醚、石油醚等非极性溶剂;(5)火炭母提取物对金黄色葡萄球菌、痢疾杆菌、枯草杆菌、藤黄球菌、白色念珠菌的最小抑菌浓度(MIC)分别为0.6g.mL-1、0.6g.mL-1、0.8g.mL-1、1.0g.mL-1、0.6g.mL-1,最小杀菌浓度(MBC)均为1.0g.mL-1。为开发新型、安全、高效的临床药物及扩大药源提供了科学依据。The antimicrobial activity of the extract from Polygonum chinense Linn.was studied in this paper.The results were as follows：（1）The antimicrobial substances existed in the stems and leaves,and antimicrobial activity of leaf extract was better than that of stem extract.（2）The antimicrobial efficiency of the extract using 65% ethanol solution was the best.（3）The antimicrobial efficiency of ultrasonic extract was better than that of Soxhlet extract,but there had a little difference.（4）The bacteriostatic test by different polar solvents showed that the antimicrobial substances easily dissolved in non-polar solvent such as ethyl ether and petroleum ether.（5）The minimal inhibitory concentrations（MIC） of the extract for Staphylococcus aureus,Shigella dysenteriae,Bacillus subtilis,Micrococcus luteus,Monilia albicans were 0.6 g·mL-1,0.6 g·mL-1,0.8 g·mL-1,1.0 g·mL-1,0.6 g·mL-1,respectively,and the minimal bactericidal concentrations（MBC） all were 1.0 g·mL-1.The results provided a basis to develop novel,safety,high-efficiency drug and extend the drug resource.

关 键 词：火炭母 提取物 抑菌活性 索氏提取 超声提取 

分 类 号：Q946[生物学—植物学] R284.2[医药卫生—中药学]