EGFR与c-Kit蛋白的表达与鼻咽癌原发瘤的进展相关  

Protein expression of EGFR and c-Kit is associated with primary tumor progression in nasopharyngeal carcinoma

作　　者：奚少彦[1,2] 吴秋良[1,2] 宗永生[3] 雍娟娟[1,2] 梁建中[1,2] 侯景辉[1,2] 卢佳斌[1,2] 

机构地区：[1]华南肿瘤学重点实验室 [2]中山大学肿瘤防治中心病理科,广东广州510060 [3]中山大学中山医学院病理学教研室,广东广州510080

出　　处：《中国病理生理杂志》2012年第4期643-648,共6页Chinese Journal of Pathophysiology

基　　金：华南肿瘤学重点实验室鼻咽癌单病种基金资助项目(No.303040736005)

摘　　要：目的:研究表皮生长因子受体(epidermal growth factor receptor,EGFR)与c-Kit蛋白2种受体酪氨酸激酶在非角化性鼻咽癌中的表达及其与临床病理的联系。方法:采用中山大学肿瘤防治中心病理科临床II期和III期非角化性鼻咽癌活检标本95例,复查临床病理资料,重新切片染色,包括HE以及EGFR、c-Kit、潜伏膜蛋白1(LMP1)和Ki-67免疫组化染色。结果:EGFR与c-Kit蛋白的阳性表达率分别为70.53%(67/95)和63.16%(60/95)。EGFR与c-Kit蛋白的表达呈正相关,有统计学意义(P<0.05)。两者在原发瘤(T)肿瘤分期中的差异均显示有统计学意义(EGFR,P<0.05;c-Kit,P<0.01)。EGFR及c-Kit蛋白的表达强度也与T分期相关(EGFR,P<0.05;c-Kit,P<0.01)。结论:非角化性鼻咽癌细胞常常表达EGFR与c-Kit蛋白。这2种受体酪氨酸激酶的表达呈正相关,且分别与鼻咽癌患者原发瘤的进展有相关性。EGFR与c-Kit蛋白可为是否需要进一步做靶向性治疗的基因分析提供理论依据。AIM： To investigate the protein expression of 2 receptor tyrosine kinases,epidermal growth factor receptor（EGFR） and c-Kit,in non-keratinizing nasopharyngeal carcinoma（NPC） and to analyze the relationship of that with the clinicopathological parameters.METHODS： Ninety-five samples of stage II and III non-keratinizing NPC biopsies were collected from the Department of Pathology,Sun Yat-sen University Cancer Center.The 10% formalin-fixed paraffin-embedded biopsy blocks were re-sectioned.Besides HE routine staining,immunohistochemistry was performed for detecting the expression of EGFR,c-Kit,latent membrane protein 1（LMP1） and Ki-67.RESULTS： The protein expression rates of EGFR and c-Kit were 70.53%（67/95） and 63.16%（60/95）,respectively.The expression score of EGFR was positively correlated with that of c-Kit protein（P0.05）.They were both correlated with T staging（EGFR,P0.05;c-Kit,P0.01）.Furthermore,the staining intensities of EGFR and c-Kit proteins were also correlated with T staging（EGFR,P0.05;c-Kit,P0.01）.CONCLUSION： The proteins of EGFR and c-Kit are usually expressed in non-keratinizing NPC cells.The immunoreactive scores of these 2 receptor tyrosine kinases are positively correlated with each other.Either the expression rate or the immunoreactive intensity of EGFR and c-Kit proteins is correlated with primary tumor progression.Immunohistochemical staining of EGFR or c-Kit protein in NPC biopsies could be recognized as an insight into further gene analysis for target therapy.

关 键 词：表皮生长因子受体 C-KIT 鼻咽肿瘤 

分 类 号：R365[医药卫生—病理学]