Aβ_(1-42)作用的小胶质细胞对体外培养的神经干细胞生存的影响  

Effects of Aβ_(1-42)-induced microglial cells on survival of neural stem cells in vitro

作　　者：韦美丹[1] 林继宗[2] 朱宁[1] 王克万[3] 王勇[1] 

机构地区：[1]南方医科大学珠江医院药剂科,广东广州510282 [2]中山大学第三附属医院肝胆外科,广东广州510630 [3]南方医科大学南方医院神经外科,广东广州510015

出　　处：《中国病理生理杂志》2012年第4期683-688,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30973162);广东省自然科学基金资助项目(No.07005203)

摘　　要：目的:探讨β-淀粉样蛋白(Aβ1-42)作用的小胶质细胞对体外培养的神经干细胞(NSCs)生存的影响。方法:利用Transwell小室在体外建立NSCs与小胶质细胞共培养体系,检测Aβ1-42蛋白作用小胶质细胞前后NSCs增殖、分化及凋亡情况。结果:与单纯共培养组相比,Aβ1-42干预共培养组的增殖率降低,且微管相关蛋白2(MAP-2)和胆碱乙酰转化酶(CHAT)阳性表达率也均降低。结论:Aβ1-42介导的炎症反应抑制了神经干细胞的增殖,促进其凋亡,并能显著降低其分化成神经元尤其是胆碱能神经元的能力。AIM： To explore the effects of β-amyloid protein 1-42（Aβ1-42）-induced microglia on the survival of cultured neural stem cells（NSCs） in vitro.METHODS： Using the Transwell chambers to build a coculture system of NSCs and microglia,we detected the proliferation,differentiation and apoptosis of the NSCs with the microglia before and after induction by Aβ1-42.RESULTS： Compared with non-intervention group,the proliferation rate of NSCs in Aβ1-42 intervention coculture group decreased,as well as the positive expression rates of microtubule-associated protein 2（MAP-2） and choline acetyltransferase.CONCLUSION： The inflammation mediated by Aβ1-42 inhib their the proliferation of NSCs and induces their apoptosis.Inflammation also significantly reduces the ratio of NSCs differentiating to neurons,especially to cholinergic neurons.

关 键 词：小胶质细胞 神经干细胞 阿尔茨海默病 淀粉样Β蛋白 

分 类 号：R363[医药卫生—病理学]