半夏总生物碱对6-OHDA诱导PC12细胞损伤的保护作用及机制  

Protective effect of total alkaloids derived from Pinellia Ternate on PC12 cells injured by 6-hydroxydopamine and its mechanism

作　　者：段凯[1] 唐瑛[2] 刁波[3] 周茜[1] 孙欢[1] 

机构地区：[1]湖北中医药大学药学院,武汉430065 [2]海军医学研究所,上海200433 [3]广州军区武汉总医院医学实验科,武汉430070

出　　处：《中国临床神经外科杂志》2012年第4期222-224,共3页Chinese Journal of Clinical Neurosurgery

摘　　要：目的探讨半夏总生物碱(TAPT)对6羟基多巴胺(6-OHDA)诱导的大鼠肾上腺嗜铬细胞瘤细胞PC12细胞株损伤的保护作用及其机制。方法通过不同剂量TAPT预处理PC12细胞后,加入6-OHDA诱导氧化应激损伤。采用甲基噻唑基四唑比色法(MTT法)检测PC12细胞活力、天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3)活性检测试剂盒测定caspase-3活性、黄嘌呤氧化酶法检测细胞总超氧化物歧化酶(T-SOD)活力,硫代巴比妥法检测丙二醛(MDA)的含量、按Fenton反应原理检测抑制羟自由基(·OH)的能力。结果浓度为100μmol/L的6-OHDA作用PC12细胞24h后,与正常对照组相比,细胞活力明显降低(P<0.05),T-SOD活性和抑制·OH的能力明显下降(P<0.05),而MDA含量和caspase-3活性明显升高(P<0.05);与6-OHDA组相比,TAPT处理组的PC12细胞活力逐渐升高(P<0.05),T-SOD活性和抑制·OH的能力明显上升(P<0.05),而MDA含量和caspase-3活性明显降低(P<0.05)。结论 TAPT对6-OHDA诱导的PC12细胞损伤具有一定的保护作用;提高T-SOD活性和抑制·OH的能力及降低MDA含量和caspase-3活性可能是其作用机制之一。Objective To explore the protective effect of total alkaloids derived from Pinellia Ternate （TAPT） on pheochromocytoma cells （PC12 cells） injured by 6-hydroxydopamin （6-OHDA） and its mechanism.Methods The oxdative stress damage to PC12 cells who were pretreated with different doses of TAPT or not was induced by 6-OHDA.The viability,total superoxide dismutase （T-SOD） activity,the content of malondialdehyde （MDA）,capability to inhibt hydroxyl free radical （·OH）,and cysteinecontaining aspartate-specific proteases 3 （caspase-3） activity in the PC 12 cells were determined.Results The viability,T-SOD activity and capability to inhibit·OH were significantly lower,and the content of MDA and caspase-3 activity were significantly higher in the PC12 cells 24 hours after the treatment with 100μmol/L 6-OHDA than those in PC12 cells of the normal group （P0.05）.The cell viability,T-SOD activity and capability to inhibit·OH were significantly higher,and the content of MDA and caspase-3 activity were significantly lower in the PC12 cells pretreated by TAPT of different doses than those in PC12 cells of the model （control） group （P0.05）.Conclusion It is suggested that TAPT may inhibit 6-OHDA-induced damage to PC12 cells via the increases in T-SOD activity and capability to inhibit·OH and the decreases in the MDA content and caspase-3 activity.

关 键 词：PC12细胞 氧化应激损伤 半夏总生物碱 羟基多巴胺 保护作用 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]