经典型霍奇金淋巴瘤EB病毒感染和p16蛋白表达的相关性研究  

The relationship of Epstein-Barr virus infection and expression of p16 protein in classic Hodgkin＇ s lymphoma

作　　者：袁轶群[1] 孙振柱[2] 

机构地区：[1]新疆维吾尔自治区石河子,石河子大学医学院,832000 [2]新疆自治区人民医院病理科

出　　处：《中国医师杂志》2012年第3期332-335,共4页Journal of Chinese Physician

摘　　要：目的探讨EB病毒在经典型霍奇金淋巴瘤（CHL）中的感染和p16蛋白表达的关系。方法应用EBER-1寡核苷酸探针以原位杂交方法检测80例CHL组织中的EBV编码的小RNA；用免疫组化方法检测p16蛋白表达。结果80例CHL患者中，男52例，女28例，男女之比为1．86：1，其中52例CHL男性患者中，EBER-1阳性率63．46％；28例CHL女性患者中，EBER-1阳性率39．29％，两者比较具有统计学意义（Х^2=4．298，P=0．038）。儿童和老年人中CHL患者共45例，其EBV表达阳性率75．56％，多于成年人的28．57％，两者比较差异具有统计学意义（Х^2=20．13，P=0．000）。EBER．1阳性表达定位在R—s细胞核上，其阳性表达率为55．00％，各亚型CHL的EBV潜伏感染检出率分别是：混合细胞型（MC）71．79％，淋巴消减型（LD）42．85％，淋巴细胞为主型（LP）47．06％，结节硬化型（NS）29．41％，且MC型明显高于NS型（Х^2=8．787，P=0．003）；p16蛋白阳性表达定位在R—S细胞的细胞核和细胞浆，其总的阳性表达率为45％，各亚型阳性率分别为：IJP47．05％，MC48．71％，NS35．29％，LD42．85％；p16蛋白与EBER-1之间呈负相关（r=-0．242，P〈0．05）。结论CHL发生可能与EBV感染有关，且p16蛋白的高表达为协助诊断CHL提供了依据。Objective To study the relationship between EBV infection of classic Hodgkin＇s lyphoma and expression of p16 protein. Methods EBER-1 and p16 protein expression in 80 cases of CHL were studied with EBER-1 oligonucleotide probe and immunohistochemistry respectively. Results 80 cases of CHL were enrolled in this study, including male 52 cases, 28 cases of female, male to female ratio of 1.86 ： 1. The positive rate of EBER-1 in female was 39. 29% and in male was 63.46% ,which have statistically significant difference （ Х^2 = 4. 298, P = 0. 038）. The positive rate of EBER-1 in children and the older was significantly higher than that in adult （Х^2 = 20. 13, P = 0. 000）. EBER-1 positive expression located in the R-S nuclei which positive expression rate was 55.00%, including mixed cell type （MC） 71.79%, lympho- cyte depletion type （LD） 42. 85%, lymphocyte predominance （LP） 47.06% and nodular sclerosis type （NS） 29.41%. MC type was significantly higher than NS （ X^2 = 8. 787, P = 0. 003 ） ;p16 was noted in the nucleus and cytoplasm. The total positive expression rate was 45%, and subtype positive rates were 47.05% （ LP）, 48.71% （ MC）, 35.29% （NS） and 42. 85% （LD）. There was a negative correlation between p16 protein and EBER-1 （ r = -0. 242, P 〈 0. 05）. Conclusions CHL may be related to EBV latent infection and it can be considered as potential markers to identify HRS cells in diagnosis.

关 键 词：霍奇金病/并发症/代谢 爱泼斯坦巴尔病毒感染/并发症/代谢 基因 p16 

分 类 号：R733[医药卫生—肿瘤] R51[医药卫生—临床医学]