低聚壳聚糖与α-丙氨酸/天冬酰胺的美拉德反应及其衍生物的抗氧化性能研究  

Maillard Reaction of Chitosan Oligosaccharide and α-Alanine/Asparagine and Antioxidant Acticity of Their Derivatives

作　　者：孙涛[1,2] 朱云[1] 谢晶[1] 薛斌[1] 毛芳[1] 

机构地区：[1]上海海洋大学食品学院 [2]上海海洋大学海洋科学研究院,上海201306

出　　处：《天然产物研究与开发》2012年第4期450-453,459,共5页Natural Product Research and Development

基　　金："十二五"国家支撑计划(2011BAD24BO2);上海市教委重点学科建设项目(J50704)

摘　　要：研究低聚壳聚糖(COS)与α-丙氨酸/天冬酰胺的美拉德反应,考察了两个体系(低聚壳聚糖的羰基与氨基的物质量比均为1∶1)的pH、吸光度和荧光值的变化。醇沉法提取低聚壳聚糖衍生物CA和CN。对两种衍生物进行红外表征和分子量测定,并研究其对超氧阳离子O2-.、DPPH自由基的清除能力以及还原能力。结果显示:抗氧化能力强弱次序为CA>CN>COS,即美拉德反应后低聚壳聚糖衍生物抗氧化能力得到显著提高,且CA的抗氧化活性优于CN,表明与小分子氨基酸进行美拉德反应制得的壳聚糖衍生物具有更好的抗氧化性。Two kinds of the chitosan oligosaccharide derivatives were prepared through Maillard reaction by heating chitosan oligosaccharide and α-alanine / asparagine（the ratio of carbonyl group and amino group was 1∶ 1）.The pH,absorbance and fluorescence were determined during the reaction.The chitosan oligosaccharide derivatives were characterized by FTIR and their molecular weight was determined by GPC.Their antioxidant activity was investigated by employing various established in vitro systems,such as superoxide,1,1-diphenyl-2-picrylhydrazyl（DPPH） radicals scavenging and reducing power.The results showed the chitosan oligosaccharide derivatives had strong antioxidant activity compared to chitosan oligosaccharide.The antioxidant activity was found to be in the order of CA CN COS.The results indicated that the derivatives prepared by chitosan oligosaccharide and α-alanine had better antioxidant activity than that prepared by chitosan oligosaccharide and asparagine.

关 键 词：低聚壳聚糖 Α-丙氨酸 天冬酰胺 美拉德反应 抗氧化性能 

分 类 号：R285[医药卫生—中药学] Q539[医药卫生—中医学]