河南鼠尾草抑制体内外α-糖苷酶活性研究  

α-Glucosidase Inhibitory Activity of Salvia honania L.H.Bailey in vivo and vitro

作　　者：闫文义[1,2] 曹乃峰[2] 张倩[2] 康文艺[2] 

机构地区：[1]河南大学医学院 [2]河南大学中药研究所,开封475004

出　　处：《天然产物研究与开发》2012年第4期533-535,共3页Natural Product Research and Development

基　　金：河南省教育厅青年骨干教师资助计划(教高2008-755)

摘　　要：本文采用96微孔板法,首次对河南鼠尾草抑制酵母和大鼠小肠α-葡萄糖苷酶活性进行研究。河南鼠尾草乙酸乙酯提取物(IC50=28.73μg/mL)和正丁醇提取物(IC50=73.90μg/mL)抑制酵母α-葡萄糖苷酶活性远高于阳性对照Acarbose(IC50=1081.27μg/mL),但只有乙酸乙酯提取物(IC50=366.79μg/mL)具有抑制大鼠小肠α-葡萄糖苷酶活性,阳性对照Acarbose未检测出其IC50。结果表明,河南鼠尾草乙酸乙酯提取物和正丁醇提取物均具有较好的酵母α-葡萄糖苷酶抑制活性,但只有乙酸乙酯提取物具有良好的大鼠小肠α-葡萄糖苷酶抑制活性。The yeast and rat intestinal α-glucosidase inhibitory activity of Salvia.honania was assayed by the method of 96-microplates for the first time.Yeast α-glucosidase inhibitory activity of ethyl acetate extracts（IC50 =28.73 μg/mL） and n-butanol extracts of S.honania（IC50 =73.90 μg/mL） were higher than that of Acarbose（IC50=1081.27 μg/mL） as positive control.Only ethyl acetate extracts（IC50 =366.79 μg/mL） showed inhibitory activity on rat intestinal α-glucosidase,and the IC50 value of Acarbose was not detected.The results indicated that both ethyl acetate extracts and n-butanol extracts of S.honania showed a better yeast α-glucosidase inhibitory activity,but only ethyl acetate extracts showed a better rat intestinal α-glucosidase inhibitory activity.

关 键 词：河南鼠尾草 酵母α-葡萄糖苷酶 大鼠小肠α-葡萄糖苷酶 抑制活性 

分 类 号：Q946.81[生物学—植物学]