胃癌外周血微小核糖核酸表达谱的初步分析  

The preliminary analysis of peripheral blood microRNA expression profile of gastric cancer

作　　者：顾玮[1] 胡梅洁[2] 孙颖[1] 王吉[1] 胡晓莹[1] 马瑾[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院卢湾分院消化科,200020 [2]上海交通大学医学院附属瑞金医院消化科

出　　处：《中华消化杂志》2012年第4期222-225,共4页Chinese Journal of Digestion

摘　　要：目的研究胃癌患者外周血微小核糖核酸（miRNA）的表达，初步建立胃癌特征性的循环miRNA表达谱，为深入研究miRNA与胃癌发生、发展并寻找新的分子标志物提供依据。方法选取6例胃癌患者和6例健康体检者，提取外周血总RNA，进行miRNA表达谱检测和生物信息学分析，采用实时定量PCR技术对芯片检测结果进行验证，对筛选出的显著差异表达miRNA的靶基因进行预测。结果胃癌组与对照组对比共有54个差异表达miRNA，其中表达上调的miRNA为35个（miRNA-504、miRNA-183、miRNA-938、miRNA-1285、miRNA-576—3p、miRNA-663等），表达下调的miRNA为19个（miRNA-433、miRNA-193b、miRNA-329、miRNA-409—3p、miRNA-154等）。实时定量PCR对其中2个上调miRNA（miRNA-504和miRNA-183）和2个下调miRNA（miRNA-433和miRNA-193b）的验证结果与芯片检测结果间具有较好的一致性。结论胃癌患者外周血中具有特异性的miRNA表达谱，这些差异表达的miRNA有可能成为新的胃癌诊断分子标志物。Objective To explore the peripheral blood microRNA （miRNA） expression of patients with gastric cancer, and to establish specific peripheral blood miRNA expression profile of gastric cancer, which would provide the evidence for investigating the role of miRNA in the genesis and development of gastric cancer and looking for new molecular markers of gastric cancer. Methods A total of 6 gastric cancer patients and 6 healthy volunteers were selected. The total RNA of peripheral blood was extracted for miRNA expression profile examination and bioinformation analysis. The results of microarray were verified by real-time PCR. The online miRNA target gene prediction software was used to predict and screen miRNA differentially expressed target genes. Results Compared with control group, there were 54 differentially expressed miRNA in gastric cancer group, of which the expression of 35 miRNA （miRNA-504, mi RNA-183, miRNA- 938, miRNA-1285, miRNA-576-3p, miRNA-663,etc） were up-regulated and 19 miRNA （miRNA-433, miRNA-193b, miRNA-329, miRNA-409-3p, miRNA-154,etc） were down-regulated. The results of real-time PCR indicated that there was a good consistency between PCR verified results and microarray results in 2 up-regulated miRNA （miRNA-504 and miRNA-183） and 2 down-regulated miRNA （miRNA-443 and miRNA-193b）. Conclusion There is specific peripheral blood miRNA expression profile of patients with gastric cancer, and these differentially expressed miRNA will likely become new diagnostic biomarkers of gastric cancer.

关 键 词：微小核糖核酸 胃肿瘤 寡核苷酸序列分析 基因表达 聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]