1株产耐热脂肪酶根霉突变株的选育  

Selection of One Rhizopus sp.Mutant Strain High Producing Thermostable Lipase

作　　者：孙春艳[1] 赵祥颖[2] 董学前[2] 乔君[2] 刘建军[1,2] 

机构地区：[1]山东农业大学食品科学与工程学院 [2]山东省食品发酵工程重点实验室,山东济南250013

出　　处：《食品与发酵工业》2012年第3期109-114,共6页Food and Fermentation Industries

摘　　要：采用紫外线、硫酸二乙酯、紫外线和硫酸二乙酯复合对实验室保藏的1株高产耐热脂肪酶的根霉SFE-L01菌株进行诱变处理,筛选获得1株突变株UD-23,产耐热脂肪酶活力比出发菌株提高了112.39%,达24 000.00 U/mL。对突变株UD-23的产酶条件及部分酶学性质进行了研究,结果表明,突变株UD-23所产脂肪酶最适作用温度为60℃,且具有良好的热稳定性。条件优化后,该突变株产酶酶活达30 000.00 U/mL,比优化前提高了25%。A high enzyme activity mutant strain UD-23 was selected from a Thermostable Lipase-producing Rhizopus SFE-L01 which was stored in our laboratory by using UV,DES and UV ＋ DES mutagenetic treatments.Compare to the original strain,the mutant＇s enzyme activity increased by 1.12 times and reached 24 000.00 U/mL.Study of the production conditions and enzymatic characteristics of the lipase from UD-23 showed that the optimal temperature of the lipase from UD-23 was 60℃.After the optimization,the enzyme activity of this mutant strain reached 30 000.00U/mL,increased by 25%.

关 键 词：耐热脂肪酶 根霉 菌种诱变 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]