1722例已婚女性宫颈细胞中人乳头瘤病毒基因的分型  

Analysis of human papillomavirus genotyping in 1722 cervical cell samples of married women

作　　者：董云灿 耿建祥[2] 张劲松[2] 王旭波[2] 李海[2] 兰建云[2] 张昶[2] 孟献威[2] 魏谨[2] 

机构地区：[1]亚能生物技术(深圳)有限公司,广东深圳518057 [2]南京中医药大学附属第三医院病理科HPV协作组,江苏南京210001

出　　处：《国际检验医学杂志》2012年第7期817-818,820,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨已婚女性宫颈细胞中23种人乳头瘤病毒(HPV)感染的基因分布情况及其临床意义。方法采用基因扩增芯片技术对1 722例已婚女性的宫颈上皮细胞标本进行23种HPV基因型别的检测,并对受检者进行相关资料分析。结果 1 722例中检出HPV感染者178例,总的HPV感染率为10.34%(178/1 722),其中单一型别的阳性检出率为8.89%(153/1 722);单一型别的感染中HPV43型为27例,其阳性检出率为1.57%(27/1 722),是最主要的感染型别。混合型HPV感染25例,其阳性检出率为1.45%(25/1 722);其中HPV16型+X型7例及16型+X型+X型2例,占混合型感染的36.00%(9/25),是混合型感染的主要型别,其次是HPV43型的2型感染6例和3型感染2例,占混合型感染的32.00%(8/25)。结论 HPV43、58型单一型别及HPV16、43型的混合型是感染的主要基因型别,基因扩增芯片检测技术可应用于宫颈细胞标本,一次性可检测23种HPV基因型别,特异性强,敏感性高。Objective To investigate the clinical distribution of 23 kinds of human papillomavirus（HPV） genotypes and its clinical significance.Methods Polymerase chain reaction and gene chip technology were utilized for the detection of 23 kinds of HPV genotypes in epithelial cell specimens from 1 722 cases of married women.And related materials of all subjects were analyzed.Results In 1 722 cases of subjects,the infection rate of HPV was 10.34%（178/1 722）.The detection rate of single genotype infection was 8.89%（153/1 722）,including 1.57%（27/1 722） for HPV43.The detection rate of mixed genotype infection was 1.45%（25/1 277）,most of which were 7 cases of HPV16＋X and 2 cases of HPV16＋X＋X,accounting for 36.00%（9/25）,and mixed infection of subtype 2 and 3 of HPV43 were in 6 and 2 cases of subjects respectively,accounting for 32.00%（8/25）.Conclusion HPV43,58,HPV16＋X or HPV16＋X＋X,HPV43＋X or HPV43＋X＋X could be the main genotypes in 1 722 cases of married women.Gene chip technology could detect multiple HPV genotypes in cervical cell samples with high sensitivity and specificity,which might be useful for pathogenesis research and prevention of cervical cancer.

关 键 词：子宫颈 人乳头瘤病毒 基因分型 基因芯片技术 

分 类 号：R737.33[医药卫生—肿瘤]