胃癌和癌前病变中树突状细胞的免疫组化研究  

Immunohistochemical study on dendritic cells in gastric mucosa of patients with gastric cancer and precancerous lesions

作　　者：罗治彬[1] 罗元辉[1] 鲁荣[1] 晋华源[1] 张朋彬[1] 徐采朴[1] 

机构地区：[1]中国人民解放军第三军医大学西南医院消化科,重庆市400038

出　　处：《世界华人消化杂志》2000年第4期400-402,共3页World Chinese Journal of Digestology

摘　　要：目的 通过研究胃粘膜中S100^+,HLA-DR^+,CD_4^+和CD_8^+产物的表达情况,以了解DC细胞在胃粘膜免疫中的作用。 方法 胃癌(143例)、慢性萎缩性胃炎(41例)、不典型增生(15例)、肠上皮化生(27例)和正常对照组(10例)共236例,都经内镜和病理诊断明确,采用HE染色、SP免疫酶标抗体组化染色法与图象分析技术相结合,观察胃粘膜中S-100^+,HLA-DR^+,CD_4^+和CD_8^+细胞数量、平均面积和平均吸光度的变化。 结果 胃癌患者胃粘膜中S-100^+(0.6±0.2)μm^2,HLA-DR^+(0.9±0.2)细胞数量明显多于正常胃粘膜(P<0.05);CD_4^+细胞(0.7±0.2)μm^2及其平均吸光度显著减少(P<0.01);CD_8^+细胞数量(1.1±0.3)μm^2、面积及其平均吸光度约有减少,但与正常组比较无显著差异(P>0.05);CD_4^+/CD_8^+比值为0.7,显著低于正常胃粘膜(P<0.01),慢性萎缩性胃炎、肠上皮化生和不典型增生胃粘膜中S-100^+分别为(1.8±0.3)μm^2,(2.2±0.6)μm^2和(2.2±0.5)μm^2,HLA-DR^+分别为(2.4±0.6)μm^2,(3.0±0.8)μm^2和(2.8±0.6)μm^2细胞数量显著增加(P<0.01);CD_8^+细胞数量分别为(1.8±0.2)μm^2,(1.9±0.2)μm^2,(1.8±0.2)μm^2均明显高于正常胃粘膜(P<0.05);CD_4^+/CD_8^+比值分别为0.8,0.6,0.6,均显著低于正常胃粘膜(P<0.05)。 结论AIM To investigate the effect of dendritic cell (DC) on gastric mucosal immunity from expressions of S100, HLA-DR, CD4 and CD8 in human gastric mucosa. METHODS A total of 236 patients had gastric cancer (GC, n = 143), chronic atrophic gastritis (CAG, n = 41), dysplasia (Dys, n = 15) and intestinal metaplasia (IM, n = 27), and normal control group (n=10). All subjects were diagnosed with endoscopy and histopathology. The number, mean area and absorbance of S100+ , HLA-DR+ , CD4+ and CD8+ cells were detected by HE and immunohistochemical (SP method) staining and image analysis.RESULTS The number of S100+ (0.6 μm2±0.2 μm2) and HLA-DR+ (0.9 μm2±0.2 μm2) cells in gastric mucosa was significantly higher in patients with GC, CAG, Dys and IM than in normal control group (P<0.05). The number of CD4+ cells (0.7 μm2±0.2 μm2) were markedly lower in GC than in normal control (2.3 Mm2±0.4 μm2) and its mean absorbance (P<0.01). The number of CD8+ cells (1.1 μm2 ±0.3 μm2) and its mean area and absorbance had no abvious difference between GC and normal (P >0.05), but the number of CD/ cells was higher in CAG, Dys and IM than in normal (P<0.05). The ratio of CD4 to CD8 was lower in GC (0.65), CAG (0.81), IM (0.59) and Dys (0.56) than in normal (1.94), P<0.05. CONCLUSION The number of DC in gastric mucosa of precancerous lesions is increased and its function is enhanced, but they are positively correlated to the degree of differentiation in gastric cancer. Inhibited function of DC in gastric mucosa is one of important factors making immunity of gastric mucosa decrease and precancerous lesions change into cancer.

关 键 词：DC细胞 胃粘膜免疫 胃肿瘤 癌前病变 

分 类 号：R735.2[医药卫生—肿瘤]