舌鳞癌细胞微环境对小鼠髓源性树突状细胞生长分化的影响  

Effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell

作　　者：童熹[1] 李勇[1] 李显[1] 王启明[1] 季平[1] 王涛[1] 

机构地区：[1]重庆医科大学口腔医学院口腔颌面外科,重庆400015

出　　处：《重庆医科大学学报》2011年第10期1172-1175,共4页Journal of Chongqing Medical University

基　　金：重庆市科委自然科学基金资助项目(编号:CSTC;2004BB5117);重庆市卫生局科研基金资助项目(编号:07-2-178);重庆市教委科学技术研究资助项目(编号:KJ090326)

摘　　要：目的:探讨舌鳞癌微环境对小鼠髓源性树突状细胞生长分化的影响。方法:小鼠骨髓前体细胞,在含重组小鼠粒细胞-巨噬细胞集落刺激因子和重组小鼠白介素-4培养体系中体外培养。实验组在上述培养液中再加入Tca8113培养上清液模拟肿瘤微环境(Tumor microenvironment,TME);对照组不加Tca8113培养上清液;镜下观察DCs形态,台酚蓝染色法计活细胞数,流式细胞术检测DCs表型(CD86、CD11c、MHC-Ⅱ)表达。结果进行LSD-t统计学分析。结果:①Tca8113微环境下可诱导出具有典型形态及表型树突状细胞;②低剂量Tca8113培养上清液微环境刺激增强刺激DCs分化成熟,中等剂量Tca8113培养上清液微环境不影响成熟DC(Mature DC,mDCs)分化,但能促进骨髓前体细胞分化为未成熟DC(Immature DC,imDC);高剂量Tca8113培养上清液微环境抑制DCs分化成熟。结论:Tca8113微环境下可成功诱导骨髓前体细胞分化为树突状细胞;舌鳞癌微环境对树突状细胞分化影响的多样性与环境中免疫因子浓度密切相关。Objective：To investigate the effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell.Methods：Marrow precursor cells were cultured in RPMI1640 medium containing rmGM-CSF and rmIL-4 ＋either cs-Tca8113 or not.Control groups were cultured without any cs-Tca8113.The morphology of DCs were observed under optical microscope;the number of survive cells was calculated by trypan blue;the phenotypes of cells were analyzed by flow cytometry.All results were analyzed statistically by LSD-t.Results：The cells cultured in tumor microenvironment appeared typical morphotype and phenotype.Microenvironment of low dose cs-Tca8113 stimulated DCs to mature.Microenvironment of medium dose cs-Tca8113 promoted bone marrow precursor to differentiate into imDC.Microenvironment of high dose cs-Tca8113 inhibited DCs to differentiate and mature.Conclusion：Bone marrow precursor cells can be successfully induced into DCs with typical characteristics in the microenvironment of Tca8113.The multiple effects of Tca8113 microenvironment on the growth and differentiation of DCs are closely related to the concentration of cs-Tca8113.

关 键 词：肿瘤微环境 树突状细胞 免疫逃逸 免疫抑制因子 

分 类 号：R739.86[医药卫生—肿瘤]