5-氮杂-2’-脱氧胞苷对涎腺腺样囊性癌中PTEN基因表达的影响  

Effects of 5-aza-2'-deoxycytidine on the expression of PTEN in adenoid cystic carcinoma cells

作　　者：张华昌[1] 向学熔[1] 范小平[2] 杨明聪[1] 吴静[1] 

机构地区：[1]重庆医科大学附属口腔医院牙周黏膜科,重庆400015 [2]重庆医科大学附属口腔医院正畸科,重庆400015

出　　处：《重庆医科大学学报》2011年第10期1194-1196,共3页Journal of Chongqing Medical University

基　　金：重庆市卫生局医学科研资助项目(编号:2009-2-140)

摘　　要：目的:研究5-氮杂-2’-脱氧胞苷(5-aza-2’-deoxycytidine,5-Aza-dc)对涎腺腺样囊性癌(Adenoid cystic carcinoma,ACC)细胞中抑癌基因第10号染色体同源丢失性磷酸酶张力蛋白基因(Phosphatase and tensin homology deleted on chromosometen,PTEN)的影响及可能的机制。方法:利用RT-PCR检测ACC细胞经甲基转移酶抑制剂5-Aza-dc作用后,PTEN基因mRNA的表达水平;Western blot检测5-Aza-dc干预对PTEN蛋白表达的影响。结果:在一定时间内,经5-Aza-dc作用后,ACC细胞中PTEN基因mRNA及蛋白表达水平逐渐增加,且差异有统计学意义(P<0.05),PTEN mRNA表达水平改变与PTEN蛋白的表达基本一致。结论:ACC细胞系中PTEN的低表达可能与PTEN基因启动子区甲基化状态有关。Objective：To detect the effects and mechanism of 5-aza-2＇-deoxycytidine on the expression of PTEN in adenoid cystic carcinoma.Methods：The expression of mRNA and protein of PTEN were analyzed by RT-PCR and western blot after treatment with methyltransferase inhibitor 5-aza-2＇-deoxycytidine（5-Aza-dc）.Results：After the treatment with methyltransferase inhibitor 5-aza-2＇-deoxycytidine（5-Aza-dc）,the levels of mRNA and protein of PTEN in adenoid cystic carcinoma cells were increased dramatically（P〈0.05）,and the levels of PTEN mRNA and protein changes were consistent.Conclusion：The hypermethylation of CpG island in promoter of PTEN is probably responsible for low PTEN expression in adenoid cystic carcinoma cells.

关 键 词：涎腺腺样囊性癌 5-氮杂-2’-脱氧胞苷 第10号染色体同源丢失性磷酸酶张力蛋白基因 

分 类 号：R782[医药卫生—口腔医学]