低氧对破骨细胞活性的影响及其机制  

Effects of hypoxia on activity of osteoclasts and its mechanism

作　　者：高文魁[1] 王伟[1] 王德元[1] 李智钢[1] 闫自强[1] 白峰[1] 

机构地区：[1]解放军第四医院骨科,青海西宁810007

出　　处：《西北国防医学杂志》2011年第6期409-412,共4页Medical Journal of National Defending Forces in Northwest China

基　　金：青海省科学技术厅科技创新能力促进计划资助项目(2010.Z.744)

摘　　要：目的:观察低氧对乳兔破骨细胞分化、活性的影响,并研究其可能的机制。方法:培养乳兔破骨细胞、成骨细胞,TRAP染色鉴定破骨细胞,比较破骨细胞在常氧(含氧量20%)及低氧(含氧量3%)条件下骨吸收陷窝面积。破骨细胞加入到第3代成骨细胞中分别于常氧和低氧条件下进行共培养,第24、48、72、96 h检测RANK、OPG、RANKL、TRAP及CtsK mRNA的表达。结果:各时间点低氧组RANK、RANKL、CtsK、TRAP mRNA表达均高于常氧组;OPG mRNA的表达低于常氧组组,具有统计学差异(P<0.05或P<0.01)。结论:低氧可激活破骨细胞TRAP和CtsK基因的上游信号,增强破骨细胞活性。Objective：To observe the effects of hypoxia on activity of osteoclasts and to investigate the possible mechanism.Methods：The osteoblasts and osteoclasts were obtained from new-laid rabbits by mechanical separation and cultured in vitro.Osteoclasts were identified by tartrate-resistant acid phosphatase（TRAP） staining.Osteoclasts resorptive pits on bone slice cultured in normoxia（20% oxygen concentration） and hypoxia（3% oxygen concentration） groups were compared.The purified osteoclasts were added to the 3 rd generation osteoblasts.The jointly cultured cells were divided into normoxia group and hypoxia group.Real-time reverse transcription PCR（RT-PCR） were used to detect the expressions of RANK,OPG,RANKL,TRAP and CtsK at 24,48,72 and 96 hours after culture.Results：Compared with normoxia group,the expression of RANK,RANKL,CtsK and TRAP mRNA were increased but the expression of OPG mRNA was decreased.Conclusion：Hypoxia could exert trophic effects on the activity of osteoclasts by activating the upstream signaling pathway of TRAP and CtsK.

关 键 词：破骨细胞 低氧 活性 机制 

分 类 号：R363[医药卫生—病理学]