5-羟色胺对体外培养肝癌细胞增殖的影响  

Effect of 5-HT on the proliferation of the cultured hepatocelluar carcinoma cells in vitro

作　　者：张冲[1] 石德红[1] 李晓林[1] 梁君蓉[1] 韩国栋[1] 李春燕[1] 

机构地区：[1]解放军第三医院消化内科,陕西宝鸡721004

出　　处：《西北国防医学杂志》2011年第6期440-442,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:探讨5-羟色胺与培养的肝癌细胞增殖的关系。方法:应用MTT法检测5-羟色胺对培养的人肝癌细胞系HCC-9204及大鼠肝癌细胞系FSK-7902增殖影响。结果:两种细胞增殖均对5-HT具有剂量和时间的依赖性。5-HT浓度在10-11至10-6mol/L之间对人肝癌细胞系HCC-9204有促增殖的作用,低于此浓度无作用,高于该浓度反而有抑制作用。同样,5-HT对大鼠肝癌细胞系FSK-7902的作用浓度为10-11至10-7 mol/L之间。结论:5-羟色胺对培养的肝癌细胞具有促进增殖的作用,这种作用具有时间及量的依赖性。Objective：To explore the relationship between 5-HT and the proliferation of cultured hepatocelluar carcinoma.Methods：MTT technique was used to analyze the effect of 5-HT with different concentrations on the proliferation of cultured human and rat hepatocelluar carcinoma cell lines.Results：5-HT has stimulation effect on the proliferation of both cell lines with dose and time dependence at the concentration ranging from 10^-12 to 10^-6 mol/L,but no effect at the concentration under 10^-12 mol/L and has inhibiting effect at the concentration more than 10^-6 mol/L.Conclusion：It suggested that 5-HT has mitogenic activity on the hepatocelluar carcinoma cells in vitro.

关 键 词：肝癌 5-羟色胺 MTT 

分 类 号：R735.7[医药卫生—肿瘤]