高糖对原代培养人肾小球系膜细胞表达MMP-9、TIMP-1的影响  

Effects of high glucose on the expression of MMP-9 and TIMP-1 in primary cultured human mesangial cells

作　　者：尹燕志[1] 牟授菡[1] 鞠建伟[1] 吕丛奎[1] 孙晓燕[1] 邓磊修 

机构地区：[1]山东烟台经济技术开发区医院,烟台264006 [2]山东先声麦得津生物制药有限公司,烟台264003

出　　处：《中国组织化学与细胞化学杂志》2011年第6期623-626,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的了解高糖对原代培养的人肾小球系膜细胞表达MMP-9、TIMP-1和MMP-9/TIMP-1的影响,进一步探讨糖尿病肾病的发病机制。方法取自愿水囊引产的胎儿肾,解剖取肾皮质剪碎,应用肾皮质组织块法合优生选择法对人肾小球系膜细胞进行原代培养。ELISA方法检测MMP-9、TIMP-1的表达变化。结果与正常组相比较,高糖组MMP-9在24h、48h、72h均显著降低(P<0.01),TIMP-1在24h、72h可显著升高(P<0.05),48h表达降低;MMP-9/TIMP-1的比值在24h、48h、72h均显著降低(P<0.01)。结论高糖能够降低MMP-9/TIMP-1,与肾小球系膜细胞细胞外基质降解能力降低密切相关。Objective To observe the effect of high glucose on the expression of MMP-9,TIMP-1 and the ratio of MMP-9/TIMP-1 in primary cultured human mesangial cells,and to elucidate their possible roles in diabetic nephropathy.Methods Kidneys isolated from voluntary induced of labor with water bag were cut into pieces,and human mesangial cells were cultured with the method of renal cortical tissue combined with eugenic selection.Then ELISA was used to measure the expression of MMP-9 and TIMP-1.Results Compared with that of the normal group,the expression of MMP-9 in the high glucose group was significantly lower at 24h,48h and 72h（P0.01）;TIMP-1was higher at 24h and 72h and lower at 48h（P 0.05）;but the ratio of MMP-9/TIMP-1 was decreased constantly at 24h,48h and 72h（P0.01）.Conclusion High glucose decreases the ratio of MMP-9/TIMP-1 in mesangial cells in vitro,which may contribute to the inhibition of ECM degradation in glomerulus.

关 键 词：人系膜细胞 原代培养 金属基质蛋白酶9 金属蛋白酶组织抑制剂1 高糖 

分 类 号：R363[医药卫生—病理学]